Biology subjects

Kelleher, E. S.

Publications and source records attributed to Kelleher, E. S..

4 recordsLinked to original sources

bioRxiv2026

NATURAL VARIATION IN RADIOTOLERANCE IS ASSOCIATED WITH DIFFERENCES IN INNATE IMMUNE ACTIVITY AND S-PHASE TRANSCRIPTION IN DROSOPHILA MELANOGASTER

Organismal tolerance of ionizing radiation is a complex trait whose genetic basis has been studied extensively, in large part due to its significance to human health and technological advancement. Conventional mutant screens in model organisms have revealed the paramount role of DNA damage response (DDR) and repair pathways in determining tolerance to ionizing radiation. However, uncovering natural genetic variation in radiotolerance is also of critical importance, as individual differences are associated with the differential susceptibility to cancer as well as differential response to radiation treatment. Genetic variation that underlies phenotype differences in natural populations often occurs in distinct genes and pathways as compared to the genes of major effect revealed by mutant screens, owing to the impact of natural selection on the former. We therefore sought to isolate natural variation in radiotolerance of Drosophila melanogaster by performing extreme QTL mapping. We generated a large genetically diverse multiparental population and exposed 3rd instar larvae to a semi-lethal dose or ionizing radiation. By sequencing surviving adults and comparing their haplotypes to unexposed controls from the same population, we identified a single major effect QTL spanning the 3rd chromosome centromere. The QTL contains 34 genes, none of which are previously implicated in radiotolerance. We interrogated the impact of these genes on radiotolerance through forward genetic analysis and RNA-seq. Our findings implicate diverse processes in radiotolerance including cell-cycle regulation and innate immune function.

genetics↗

bioRxiv2021

Natural tolerance to transposition is associated with Myc-regulation and DNA repair

Transposable elements (TE) are mobile genetic parasites whose unregulated activity in the germline causes DNA damage and sterility. While the regulation of TE mobilization by hosts is studied extensively, little is known about mechanisms that could allow germline cells to persist in the face of genotoxic stress imposed by active transposition. Such tolerance mechanisms are predicted to be beneficial when new TEs invade and host repression has not yet evolved. Here we use hybrid dysgenesis--a sterility syndrome of Drosophila caused by transposition of invading DNA transposons--to uncover genetic variants that confer tolerance to transposition. Using a panel of highly recombinant inbred lines of Drosophila melanogaster, we identified two linked quantitative trait loci (QTL), that determine tolerance in young and old females, respectively. Through transcriptomic and phenotypic comparisons, we provide evidence that young tolerant females exhibit enhanced repair of double-stranded breaks, explaining their ability to withstand high germline transposition rates. We furthermore identify the germline differentiation factor brat as an independent tolerance factor, whose activity may promote germline maintenance in aging dysgenic females. Together, our work reveals the diversity of potential tolerance mechanisms across development, as well as tolerant variants that may be beneficial in the context of P-element transposition.

genomics↗

bioRxiv2017

p53 is required for female germline stem cell maintenance in P-element hybrid dysgenesis.

Hybrid dysgenesis is a sterility syndrome resulting from the mobilization of certain transposable elements in the Drosophila germline. Particularly extreme is the hybrid dysgenesis syndrome caused by P-element DNA transposons, in which dysgenic female ovaries often contain few or no germline cells. Those offspring that are produced from dysgenic germlines exhibit high rates of de novo mutation and recombination, implicating transposition-associated DNA damage as the cause of germline loss. However, how this loss occurs, in terms of the particular cellular response that is triggered (cell cycle arrest, senescence, or cell death) remains poorly understood. We demonstrate that two components of the DNA damage response, Checkpoint kinase 2 and its downstream target p53, are determine the frequency of ovarian atrophy that is associated with P-element hybrid dysgenesis. We further show that p53 is strongly induced in the germline stem cells (GSCs) of dysgenic females, and is required for their maintenance. Our observations support the critical role for p53 in conferring tolerance of transposable element activity in stem cells.

genetics↗

bioRxiv2017

The Evolution Of Small RNA-Mediated Silencing Of An Invading Transposable Element

Transposable elements (TEs) are genomic parasites that impose fitness costs on their hosts by producing deleterious mutations and disrupting gametogenesis. Host genomes avoid these costs by regulating TE activity, particularly in germline cells where new insertions are heritable and TEs are exceptionally active. However, the capacity of different TE-associated fitness costs to select for repression in the host, and the role of selection in the evolution of TE regulation more generally, remain controversial. In this study, we use forward, individual-based simulations to examine the evolution of small-RNA-mediated TE regulation, a conserved mechanism for TE repression that is employed by both prokaryotes and eukaryotes. To design and parameterize a biologically realistic model, we drew on an extensive survey of empirical studies of the transposition and regulation of P-element DNA transposons in Drosophila melanogaster. We observed that even under conservative assumptions, where small-RNA-mediated regulation reduces transposition only, repression evolves rapidly and adaptively after the genome is invaded by a new TE. We further show that the spread of repressor alleles is greatly enhanced by two additional TE-imposed fitness costs: dysgenic sterility and ectopic recombination. Finally, we demonstrate that the mutation rate to repression (i.e., the size of the mutational target) is a critical parameter that influences both the evolutionary trajectory of host repression and the associated proliferation of TEs after invasion. Our findings suggest that adaptive evolution of TE regulation may be stronger and more prevalent than previously appreciated, and provide a framework for evaluating empirical data.

evolutionary biology↗