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Biology subjects

Karavolias, N.

Publications and source records attributed to Karavolias, N..

2 recordsLinked to original sources

Engineering compact Physalis peruviana (goldenberry) to promote its potential as a global crop

SOCIETAL STATEMENTEN - Goldenberry (Physalis peruviana) produces sweet, nutritionally-rich berries, yet like many minor crops, is cultivated in limited geographical regions and has not been a focus of breeding programs for trait enhancement. Leveraging knowledge of plant architecture-related traits from related species, we used CRISPR/Cas9-mediated gene editing to generate a compact ideotype to advance future breeding efforts and agricultural production. Goldenberry growers will benefit from these compact versions because it optimizes per plot yield, facilitating larger-scale production to meet rising consumer popularity and demand. SP - La uchuva (Physalis peruviana) produce frutos dulces y ricos en nutrientes, pero, igual que muchos cultivos minoritarios, se cultiva en zonas geograficas limitadas y no ha sufrido un proceso de mejora. Aprovechando conocimientos sobre rasgos relacionados con la arquitectura vegetal de especies relacionadas, hemos usado edicion genica mediante CRISPR/Cas9 para generar un ideotipo compacto para promover futuros esfuerzos en su mejora y en produccion agricola. Los productores de uchuva se podran beneficiar de estas versiones compactas ya que optimiza el rendimiento por parcela, facilitando asi la produccion a una mayor escala para cubrir la creciente popularidad y demanda de los consumidores.

plant biology↗

CRISPR/Cas9 knockout of EPFL10 reduces stomatal density while maintaining photosynthesis and enhancing water conservation in rice

Rice (Oryza sativa) is of paramount importance for global nutrition, supplying at least 20% of global calories. However, water scarcity and increased drought severity are anticipated to reduce rice yields globally. Rice stomatal developmental genetics were explored as a mechanism to improve drought resilience while maintaining yield under climate stress. CRISPR/Cas9-mediated knockouts of EPFL10 and STOMAGEN yielded lines with c. 80% and 25% of wild-type stomatal density, respectively. epfl10 lines with moderate reductions in stomatal densities are able to conserve water to similar extents as stomagen lines, but do not suffer from the concomitant reductions in stomatal conductance, carbon assimilation, or thermoregulation observed in stomagen knockouts. Moderate reductions in stomatal densities achieved by editing EPFL10 may be a climate-adaptive approach in rice that can safeguard yield. Editing the paralog of STOMAGEN in other species may provide a means to tune stomatal density in agriculturally important crops beyond rice.

plant biology↗