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Biology subjects

Kanazawa, H.

Publications and source records attributed to Kanazawa, H..

3 recordsLinked to original sources

Synthesising robot behaviour through reinforcement learning for homeostasis

Homeostasis is a fundamental property for the survival of animals. Computational reinforcement learning provides a theoretically sound framework for learning autonomous agents. However, the definition of a unified motivational signal (i.e., reward) for integrated survival behaviours has been largely underexplored. Here, we present a novel neuroscience-inspired algorithm for synthesising robot survival behaviour without the need for complicated reward design and external feedback. Our agent, the Embodied Neural Homeostat, was trained solely with feedback generated by its internal physical state and optimised its behaviour to stabilise these internal states: homeostasis. To demonstrate the effectiveness of our concept, we trained the agent in a simulated mechano-thermal environment and tested it in a real robot. We observed the synthesis of integrated behaviours, including walking, navigating to food, resting to cool down the motors, and shivering to warm up the motors, through the joint optimisation for thermal and energy homeostasis. The Embodied Neural Homeostat successfully achieved homeostasis-based integrated behaviour synthesis, which has not previously been accomplished at the motor control level. This demonstrates that homeostasis can be a motivating principle for integrated behaviour generation in robots and can also elucidate the behavioural principles of living organisms.

neuroscience↗

Flow zoometry of Drosophila

Drosophila serves as a highly valuable model organism across numerous fields including genetics, immunology, neuroscience, cancer biology, and developmental biology. Central to Drosophila-based biological research is the ability to perform comprehensive genetic or chemical screens. However, this research is often limited by its dependence on laborious manual handling and analysis, making it prone to human error and difficult to discern statistically significant or rare events amid the noise of individual variations resulting from genetic and environmental factors. In this article we present flow zoometry, a whole-animal equivalent of flow cytometry for large-scale, individual-level, high-content screening of Drosophila. Our flow zoometer automatically clears the tissues of Drosophila melanogaster, captures three-dimensional (3D) multi-color fluorescence tomograms of single flies with single-cell volumetric resolution at an unprecedented throughput of over 1,000 animals within 48 hours (24 hr for clearing; 24 hr for imaging), and performs AI-enhanced data-driven analysis - a task that would traditionally take months or years with manual techniques. To demonstrate its broad applications, we employed the flow zoometer in various laborious screening assays, including those in toxicology, genotyping, and tumor screening. Flow zoometry represents a pivotal evolution in high-throughput screening technology: previously from molecules to cells, now from cells to whole animals. This advancement serves as a foundational platform for "statistical spatial biology", to improve empirical precision and enable serendipitous discoveries across various fields of biology.

bioengineering↗

A neuromodulatory model for determining the effect of emotion-respiration-cognition coupling on the time-to-respond

Respiration and emotional stimuli modulate cognitive ability and the reaction time to generate bodily movement. To understand mechanisms for emotion-respiration-cognition coupling, first, we considered a schematic feed-forward neural network, in which neurons was biased by respiratory-relevant sensory input and the activation function of a neuron was modulated by a neuromodulator, such as norepinephrine (NE). Furthermore, we assumed that the neural model received a stimulus input and generated a response action upon the activity of the output neuron exceeding a certain threshold. Time-to-respond (TTR) was equivalently modulated by the intensity of the input bias and the neuromodulator strength for small action execution threshold; however, it was dominantly modulated by only the neuromodulator for high threshold. Second, we implemented a comprehensive model comprising a cardio-respiration relevant neuromechanical-gas system, a respiratory central pattern generator (CPG), NE dynamics to modulate neurocognitive dynamics, and a locus coeruleus (LC) circuit, which was the primary nucleus for controlling NE. The LC neurons received pCO2 or synaptic current from an inspiratory neurons, which resulted in shortened TTR by a stimulus input during inhalation. By contrast, upon receiving pulmonary stretch information, the TTR was shortened by a stimulus input during exhalation. In humans, TTR is shortened when a fear-related stimulus is presented during inhalation, and likewise, TTR is weakly-shortened when surprise-related stimulus is presented during exhalation. Hence, we conclude that emotional stimuli in humans may switch the gating strategies of information and the inflow to LC to change the attention or behavior strategy.

neuroscience↗