Kalyuzhnyy, V. | Search bioRxiv

bioRxiv2026

Deep behavioral phenotyping of pathogen infected mosquitoes reveals species-specific behavior changes enhancing transmission

Mosquito-borne pathogens are often proposed to alter mosquito behavior to enhance transmission, yet the prevalence, magnitude, and consequences of such effects remain unclear. Using custom high- throughput behavioral assays and deep-learning analysis, we quantified the blood-feeding behavior of more than 5,000 mosquitoes, and long-term activity patterns of more than 1,000 mosquitoes across six clinically relevant mosquito-pathogen combinations. Infection effects were highly species-specific. Anopheles stephensi infected with an African Plasmodium falciparum strain showed elevated flight activity throughout infection, an increased tendency to blood-feed upon host contact, and prolonged feeding times. In contrast, infection with an Asian P. falciparum strain or P. vivax had little impact on An. stephensi behavior. We detected no behavioral changes associated with P. falciparum infection in Anopheles gambiae s.s. or Anopheles coluzzii. Interestingly, dengue virus infection in Aedes aegypti increased landing, probing, and engorgement rates. Together, these results demonstrate that infection-induced behavioral change is not a general phenomenon among mosquito-borne pathogens, yet differs markedly per mosquito and pathogen species. A transmission model parameterized with our data showed that even modest behavioral shifts can substantially increase transmission, underscoring the potential epidemiological importance of subtle phenotypic shifts.

animal behavior and cognition↗

bioRxiv2025

Blood, sweat, and beers: investigating mosquito biting preferences amidst noise and intoxication in a cross-sectional cohort study at a large music festival

IntroductionWe live in a world split between mosquito magnets and those lucky enough to remain (nearly) untouched. The reasons why some people attract more mosquito bites than others remain largely mysterious. In the Mosquito Magnet Trial, we investigated differences in mosquito attraction amongst festivalgoers with varying levels of hygiene and intoxication. MethodsOur study was conducted in a slightly steamy pop-up laboratory inside four connected shipping containers at Lowlands Festival in The Netherlands (August 18-20, 2023). Participants completed an anonymous questionnaire on hygiene, diet, and festival-related behaviour (including alcohol uptake and shared sleeping arrangements). Mosquito attraction was measured using a custom designed setup: a transparent cage with perforations where female Anopheles mosquitoes were offered a choice between a sugar-feeder and the participants arm. Mosquitoes could only smell, not bite, the participants arm. Attraction was quantified through video imaging, measuring arm landings relative to total landings. Mosquito attraction was correlated with questionnaire responses and skin microbiota profiles collected from forearm skin swabs. ResultsAmongst the 465 included participants, mosquitoes showed a clear fondness for those who drank beer over those who abstained from the liquid gold (Fold Change 1.35, 95% CI 1.12-1.63, PFDR<0.001). Attraction was also contagious: participants that successfully lured a fellow human into their tent the previous night also proved more enticing to mosquitoes (FC 1.34, 95% CI 1.14-1.58, PFDR=0.002). Meanwhile, skipping the morning showering routine and using sunscreen reduced mosquito attraction (FC 0.52, 95% 0.38-0.70, PFDR<0.001). Streptococci were more abundant on the skin of highly attractive individuals (Puncorrected=0.017), and the overall abundance of malodour associated bacteria was high - possibly reflecting the containers smell. ConclusionThe Mosquito Magnet Trial, to our knowledge the largest study of its kind, was conducted in a loosely controlled setting with a selection bias towards science loving festivalgoers. That said, using our custom designed experimental set-up, we found that mosquitoes are drawn to those who avoid sunscreen, drink beer, and share their bed. They simply have a taste for the hedonists among us.

animal behavior and cognition↗