Justino, A. B. | Search bioRxiv

bioRxiv2023

Not only age affects cardiovascular parameters, salivary biomarkers, and their correlation, but the level of physical conditioning changes this behavior in the elderly

To compare the physical conditioning, hemodynamic, and salivary biomarkers between elderly athletes and the physically active elderly. 14 men: EA (n = 8) and PAE (n = 6). Collection times (T0; TE; T5; T15). A negative correlation was found between SF and cardiovascular parameters, BL, and STP in both groups, but this was almost double among PAE. For HR and SBP, there was a faster recovery in EA. The EA increase was correlated with SBP, while for PAE it was correlated with HR. BL showed an increase in TE, reaching 481% in EA and 639% in PAE. SNO showed a similar increase for the groups at the TE, but at T5, while EA already showed a reduction, PAE saw a 94% increase, with a slower decay for this group at T15. The SF presented the negative {Delta}% was almost double in PAE, with a quick recovery already at T5 for EA and levels still negative at all times for PAE. For SIgA-s, there was an increase of 37% in EA and only 7% at the TE in PAE; 41% in EA and 15% in PAE for T5; and 26% in EA and 14% in PAE at T15. SA showed a higher peak in EA (TE) and less acute in PAE (T5) but there was a decrease among both at T15. STP increased by 126% in EA and 438% in PAE, already showing a return at T5 for EA, but increasing by 213% in PAE. Negative levels were reached at T15 for EA but levels remained high in PAE. Levels of physical conditioning affect cardiovascular parameters, salivary biomarkers, and their correlation within the over-60s.

physiology↗

bioRxiv2021

Alkaloid and acetogenin-rich fraction from Annona crassiflora fruit peel inhibits proliferation and migration of human liver cancer HepG2 cells

Plant species from Annonaceae are commonly used in traditional medicine to treat various cancer types. This study aimed to investigate the antiproliferative potential of an alkaloid and acetogenin-rich fraction from the fruit peel of Annona crassiflora in HepG2 cells. A liquid-liquid fractionation was carried out on the ethanol extract of A. crassiflora fruit peel in order to obtain an alkaloid and acetogenin-rich fraction (AF-Ac). Cytotoxicity, proliferation and migration were evaluated in the HepG2 cells, as well as the proliferating cell nuclear antigen (PCNA), vinculin and epidermal growth factor receptor (EGFR) expression. In addition, intracellular Ca2+ was determined using Fluo4-AM and fluorescence microscopy. First, 9 aporphine alkaloids and 4 acetogenins that had not yet been identified in the fruit peel of A. crassiflora were found in AF-Ac. The treatment with 50 {micro}g/mL AF-Ac reduced HepG2 cell viability, proliferation and migration (p < 0.001), which is in accordance with the reduced expression of PCNA and EGFR levels (p < 0.05). Furthermore, AF-Ac increased intracellular Ca2+ in the HepG2 cells, mobilizing intracellular calcium stores, which might be involved in the anti-migration and anti-proliferation capacities of AF-Ac. Our results support the growth-inhibitory potential of AF-Ac on HepG2 cells and suggest that this effect is triggered, at least in part, by PCNA and EGFR modulation and mobilization of intracellular Ca2+. This study showed biological activities not yet described for A. crassiflora fruit peel, which provide new possibilities for further in vivo studies to assess the antitumoral potential of A. crassiflora, especially its fruit peel.

biochemistry↗