Biology subjects

Jonvel, R.

Publications and source records attributed to Jonvel, R..

3 recordsLinked to original sources

bioRxiv2025

A minimal hybridization capture system for the parallel enrichment and cost-effective detection of ancient human pathogens

The preservation of ancient DNA in archaeological remains enables identification of past disease agents. However, pathogen DNA is typically highly diluted by host and environmental DNA, limiting detection. Here, we present a proof-of-concept study using in-solution hybridization capture to improve detectability of a pre-defined set of 12 pathogens. We validate the method by detecting Yersinia pestis, the plague agent, in six individuals from 17th and 18th century French plague cemeteries with minimal sequencing. Expanding our probe set to target biomarkers of virtually any pathogen of interest offers a powerful tool for tracking the prevalence of infectious diseases in ancient populations.

molecular biology↗

bioRxiv2022

Dental pulp metagenomics reveals ancient human Herpesviridae.

Following a recent report on ancient human Herpesviridae (HHV), we adapted a standard protocol used for the high-throughput sequencing of modern DNA to recover ancient DNA (aDNA) from dental pulp specimens collected at two historical burial sites. Investigation of 36 soldiers buried in Sevastopol, Crimea, during the 1853-1856 Crimean War yielded genomic sequences covering 8.5% of the herpes simplex virus (HSV)-1 genome, 15.7% of the HSV-2 genome, 1.9% of the Epstein-Barr virus (EBV) genome and 3.9% of the cytomegalovirus genome. Further investigation of 16 civilians buried in the 17th century in Amiens, France, yielded genomic sequences covering 9.8% of the HHV-1 genome and 2.2% of the HHV-6A genome; all data obtained with parallel negative controls was appropriately negative. Specific qPCRs confirmed the presence of four HSV-1, two HSV-2 and one EBV sequences in Sevastopol and two HSV-1 and one HHV-6 sequences in Amiens. HHV-6 was further detected by fluorescent in situ hybridization. These results demonstrated the usefulness of modern metagenomic protocols for high-throughput sequencing of aDNA in dental pulp.

microbiology↗

bioRxiv2020

Five millennia of Bartonella quintana bacteremia.

Bartonella quintana caused trench fever in the framework of two World Wars and is now recognized as an agent of re-emerging infection. Many reports indicated the popularity of B. quintana exposure since the 90s. For evaluating its prevalence in ancient populations, we used real-time PCR to detect B. quintana DNA in 400 teeth collected from 145 individuals dating from the 1st to 19th centuries in nine archeological sites with the presence of negative controls. Fishers exact test was used to compare the prevalence of B. quintana detection in civil and military populations. B. quintana DNA was confirmed in a total of 28/145 (19.3%) individuals, comprising 78 citizens and 67 soldiers, 20.1% and 17.9% of which were positive for B. quintana bacteremia, respectively. This study collected previous studies on these ancient samples and showed that the presence of B. quintana infection followed the course of time in human history; a total of 14/15 sites from five European countries had a positive prevalence. The positive rate in soldiers was higher than those of citizens, with 20% and 18.8%, respectively, in the 18th - 19th centuries, but the difference in frequency was not significant. These results confirmed the role of dental pulp in diagnosing B. quintana bacteremia in ancient populations and showed the incidence of B. quintana in both citizens and soldiers. Many recent findings contributed to understanding the coevolution of the relationship between B. quintana and humans.

microbiology↗