Biology subjects

Jaindl, G.

Publications and source records attributed to Jaindl, G..

3 recordsLinked to original sources

bioRxiv2026

Measuring neuronal input-output operations in vivo with random access projection microscopy

Neurons transform thousands of synaptic inputs into spiking output, and understanding this transformation requires measuring many submicron synapses at millisecond timescales in vivo. Here we present SLAP2, a two-photon random access projection microscope with no per-target access time cost, yielding over 5-fold faster imaging than existing methods, and performant real-time hardware motion correction. Flexible scanning modes enable measurement of hundreds of synapses at ~200 Hz, >800 synapses volumetrically at 50-100 Hz, and compartment-resolved dendritic voltage at 2-10 kHz in behaving mice. Simultaneous dendritic glutamate and somatic calcium imaging in visual cortex pyramidal neurons revealed nonlinear input-output amplification of stimulus onset and preferred stimulus orientation responses. Amplified features are associated with spatially organized inputs and stronger trial-to-trial coupling between synaptic activity and somatic output. These measurements establish SLAP2 as a powerful tool for recording intricate patterns of subcellular fluorescence dynamics in behaving animals.

neuroscience↗

bioRxiv2026

Raster photostimulation of large-scale neural populations

Neural computations are implemented by distributed neural populations that typically span multiple brain areas. Simultaneous recording and photo-activation experiments can reveal the structure and logic of these neural computations, but such methods are typically limited to small subsets of neurons in restricted fields of view. Here we describe a new system called raster photostimulation for activating and recording thousands of neurons, over a short 300 ms time window and over a large 5 mm field-of-view on a two-photon mesoscope. The photo-activation is precisely matched to the neural recording configuration, as it uses the same optical path, although with a different laser that is independently gated. We demonstrate pixel-level precision, frame-by-frame mask updating, and single-frame photostimulation of thousands of neurons. While this method lacks the precise temporal control of alternative methods, it compensates with ease-of-use, spatial precision, cost of implementation and by pushing the limits on the number of near-simultaneously stimulated neurons.

neuroscience↗

bioRxiv2024

Grayscale 4D Biomaterial Customization at High Resolution and Scale

Hydrogel biomaterials have proven indispensable for three-dimensional (3D) cell culture but have fallen short in replicating the innate physiochemical complexity of real tissue. Though traditional photolithography affords localized material manipulation, robust methods that govern when, where, and to what extent such phototailoring occurs throughout materials would be profoundly enabling towards fabricating more-realistic 3D tissue constructs. Here, we introduce "grayscale image z-stack-guided multiphoton optical-lithography" (GIZMO) as a generalizable and intuitive strategy to rapidly photomodulate materials in full 3D non-binary patterns at submicron resolutions spanning large volumes (>mm3). Highlighting its versatility, we employ GIZMO to variably photopattern biomolecule release from, protein immobilization to, and degradation within hydrogels based on biologically derived or synthetic grayscale image stacks with unprecedented complexity. We anticipate that GIZMO will enable new opportunities to probe and manipulate cell fates, as well as to engineer complex functional tissue.

bioengineering↗