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Biology subjects

J, A.

Publications and source records attributed to J, A..

2 recordsLinked to original sources

Characterization of Onion Seedlots into Different Storability Groups with 4 Parameter Hill Function (4-PHF)

Onion varieties were classified into different storability groups by comparing two approaches (i)germination and vigor indices (conventional germination parameters) (ii)variables extracted from 4 Parameter Hill Function (4-PHF), after mimicking ageing conditions with accelerated ageing (42 {degrees}C and 100% RH). The study revealed that in comparison to evaluation using conventional germination parameters, the parameters extracted using 4-PHF provided realistic characterization of varieties as good, medium and poor storers. Time related parameters like time to maximum germination rate (TMGR), time to 50% germination (T50), difference between time at germination onset (lag) and 50% germination (Dlag-50), uniformity (U) along with germination percent (a) and area under curve (AUC) were decisive in identification of the varieties to a storage category which was misinterpreted with exclusive use of conventional approach. The distinction between good and medium storers was not of much significance but shift of varieties like Bhima Super, Pusa Red and Agrifound Light Red from poor to good performance cluster could be detected exclusively through 4-PHF analysis. Curve fittings highlighted AUC as the most crucial parameter contributing towards clustering of the varieties in different storability groups. Our study is the first reported research of using 4-PHF mathematical function for seedlot characterization into different storability groups.

plant biology↗

FRET based aptamer assay for sensitive detection of Salmonella paratyphi A and revealing its molecular interaction with DNA gyrase: An in silico accessment

Rapid pathogen detection and identification of its serovars are crucial to provide essential treatment during pandemic circumstances. Herein, we developed a facile and versatile FRET-based aptasensor for rapid Salmonella paratyphi A detection. The ssDNA aptamers specific towards pathogenic Salmonella paratyphi A were generated via whole-cell SELEX. The aptamer was conjugated onto quantum dot (QD) that served as the molecular beacon and graphene oxide (GO) was used as fluorescence quencher. The detection of Salmonella paratyphi A leads to the quenching of QD fluorescence due to the non-covalent interaction between GO and CdTe quantum dot. The assay shows a detection limit up to 10 cfu{middle dot}mL-1 with no cross-reactivity towards closely related species. The spiking analysis demonstrated an inter-assay coefficient of variance less than 8 % and recovery rate between 85%-102% mitigates assay reliability. Further analysis with commercially available ELISA kit validated the reliability of the developed aptasensor. Furthermore, molecular dynamics simulation was used to establish the mechanism of action of generated aptamer against bacterial DNA gyrase protein. The strong non-bonded interaction energies along with hydrogen bonds between the aptamer and protein inhibit the function of the bacteria.

synthetic biology↗