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Iqbal, S.

Publications and source records attributed to Iqbal, S..

2 recordsLinked to original sources

Wearable Eye-tracking for Research: Automated dynamic gaze mapping and accuracy/precision comparisons across devices

Wearable eye-trackers offer exciting advantages over screen-based systems, but their use in research settings has been hindered by significant analytic challenges as well as a lack of published performance measures among competing devices on the market. In this article, we address both of these limitations. We describe (and make freely available) an automated analysis pipeline for mapping gaze data from an egocentric coordinate system (i.e. the wearable eye-tracker) to a fixed reference coordinate system (i.e. a target stimulus in the environment). This pipeline allows researchers to study aggregate viewing behavior on a 2D planar target stimulus without restricting the mobility of participants. We also designed a task to directly compare calibration accuracy and precision across 3 popular models of wearable eye-trackers: Pupil Labs 120Hz Binocular glasses, SMI ETG 2 glasses, and the Tobii Pro Glasses 2. Our task encompassed multiple viewing conditions selected to approximate distances and gaze angles typical for short- to mid-range viewing experiments. This work will promote and facilitate the use of wearable eye-trackers for research in naturalistic viewing experiments.

neuroscience

Screening, Characterization and Optimization of antibacterial peptides, produced by Bacillus safensis strain MK-12 isolated from waste dump soil KP, Pakistan

AimsThe current study was designed to isolate, screen and identify the indigenous soil antibacterial exhibiting bacteria (AEB) and effect of various parameters on growth of AEB and antibacterial peptides production.\n\nMethods and resultsThe soil isolates were screened for antagonistic activity against a set of ATCC and local MDR human pathogenic bacterial strains. The antibacterial compound was protein in nature, exhibited no haemolysis and molecular weight was less than 20 KDa. The potential AEB isolate was identified by morphology, biochemical testing and by 16S rRNA gene sequencing as B. safensis MK-12. Growth and antibacterial activity was optimized for B. safensis strain MK-12, exhibited maximum growth as well as antibacterial activity after 48 hours of incubation at pH 8, 30 {degrees}C in shaking incubator when fermented in optimized medium.\n\nConclusionThe current study results indicate that indigenous soil is rich source of AEB and could be a promising source of antimicrobial compounds to fight against MDR bacteria in future.\n\nSignificance and impactThis is the first scientific report on soil bacteria from northern region of Pakistan as per our knowledge. Therefore, screening of soil bacteria for antibacterial activity from unexplored area may contribute towards new antibiotic. Selected soil strain in the current study exhibited promising antibacterial activity against human pathogenic MDR bacterial strains.

microbiology