Biology subjects

Huard-Chauveau, C.

Publications and source records attributed to Huard-Chauveau, C..

3 recordsLinked to original sources

bioRxiv2026

The root knot nematode effector MiEFF186 interacts with a splicing factor to reshape plant transcriptome

The root-knot nematode Meloidogyne incognita secretes specific effectors to manipulate root cells and induce their redifferentiation into multinucleate feeding giant cells, which are essential for its development. A dedicated screen identified the M. incognita MiEFF186a effector as a regulator of alternative splicing, a crucial mechanism of transcriptional gene regulation whereby a single gene can produce multiple mature mRNA variants, in plants. EFF186 is expressed in the esophageal glands of nematode juveniles, and in planta, it localizes to the nucleus and accumulates in the nucleolus. A yeast two-hybrid approach identified the nucleolar G-patch protein GDP1/CIS2 as a MiEFF186a target in tomato and Arabidopsis. Transcriptomic analyses of Arabidopsis roots ectopically expressing MiEFF186a or impaired in GDP1/CIS2 function revealed changes in both gene expression and alternative splicing, with partially overlapping responses. Finally, alteration of GDP1/CIS2 expression by virus-induced gene silencing in Solanaceae or in Arabidopsis gdp1 mutants affects susceptibility to M. incognita. Together, these results identify GDP1/CIS2 as a host target of MiEFF186a and support a role for these proteins in the modulation of host RNA metabolism during nematode parasitism.

plant biology↗

bioRxiv2024

The Arabidopsis leucine rich repeat receptor-like kinase MIK2 interacts with RKS1 and participates to the control of quantitative disease resistance to the bacterial pathogen Xanthomonas campestris

Molecular mechanisms underlying qualitative resistance have been intensively studied. In contrast, although quantitative disease resistance (QDR) is a common, durable and broad-spectrum form of immune responses in plants, only a few related functional analyses have been reported. In this context, the atypical kinase RKS1 is a major actor of QDR to the bacterial pathogen Xanthomonas campestris (Xcc) and is positioned in a robust protein-protein decentralized network. Among the putative interactors of RKS1 found by yeast two hybrid screening, we identified the receptor like kinase MDIS1-Interacting Receptor-like Kinase 2 (MIK2). Here, by multiple and complementary strategies including protein-protein interaction tests, mutant analysis and network reconstruction, we report that MIK2 is a component of RKS1 mediated QDR to Xcc. First, by co-localization experiment, co-immunoprecipitation (Co-IP) and Bimolecular Fluorescence Complementation (BiFC), we validated the physical interaction between RKS1 and MIK2 in the plasma membrane. Using mik2 mutants, we then showed that MIK2 is required for QDR at the same level as RKS1. Interestingly, a catalytic mutant of MIK2 was able to interact with RKS1 but unable to fully complement the mik2-1 mutant in response to Xcc. Finally, we investigated a potential role of the MIK2-RKS1 complex as a scaffolding component for coordination of perception events, by constructing a RKS1-MIK2 centered protein-protein network. Eight mutants corresponding to seven RLKs of this network showed a strong and significant alteration in QDR to Xcc. Our findings provide new insights into the molecular mechanisms underlying perception events involved in QDR to Xcc.

plant biology↗

bioRxiv2018

In situ relationships between microbiota and potential pathobiota in Arabidopsis thaliana

A current challenge in microbial pathogenesis is to identify biological control agents that may prevent and/or limit host invasion by microbial pathogens. In natura, hosts are often infected by multiple pathogens. However, most of the current studies have been performed under laboratory controlled conditions and by taking into account the interaction between a single commensal species and a single pathogenic species. The next step is therefore to explore the relationships between host-microbial communities (microbiota) and microbial members with potential pathogenic behavior (pathobiota) in a realistic ecological context. In the present study, we investigated such relationships within root and leaf associated bacterial communities of 163 ecologically contrasted Arabidopsis thaliana populations sampled across two seasons in South-West of France. In agreement with the theory of the invasion paradox, we observed a significant humped-back relationship between microbiota and pathobiota -diversity that was robust between both seasons and plant organs. In most populations, we also observed a strong dynamics of microbiota composition between seasons. Accordingly, the potential pathobiota composition was explained by combinations of season-specific microbiota OTUs. This result suggests that the potential biomarkers controlling pathogens invasion are highly dynamic.

microbiology↗