Huang, M. A. | Search bioRxiv

bioRxiv2021

Myofibroblast transcriptome indicates SFRP2+ fibroblast progenitors in systemic sclerosis skin

Skin and lung fibrosis in systemic sclerosis (SSc) is driven by myofibroblasts, alpha-smooth muscle actin expressing cells that arise from a variety of cell types in murine fibrosis models. Utilizing single cell RNA-sequencing to examine the transcriptome changes, we show that SSc dermal myofibroblasts arise from an SFRP2/DPP4-expressing progenitor fibroblast population that globally upregulates expression of transcriptome markers, such as PRSS23 and THBS1. Only a fraction of SSc fibroblasts differentiate into myofibroblasts, as shown by expression of additional markers, SFRP4 and FNDC1. The myofibroblast transcriptome implicates upstream transcription factors that drive myofibroblast differentiation.

cell biology↗

bioRxiv2018

The emotional valence and features of subliminal priming images impact conscious perception of facial expressions

We investigated, in young healthy subjects, how the affective content of subliminally presented priming images and their specific visual attributes impacted conscious perception of facial expressions. The priming images were broadly categorised as aggressive, pleasant, or neutral and further subcategorised by the presence of a face and by the centricity (egocentric or allocentric vantage-point) of the image content. Subjects responded to the emotion portrayed in a pixelated target-face by indicating via key-press if the expression was angry or neutral. Priming images containing a face compared to those not containing a face significantly impaired performance on neutral or angry target-face evaluation. Recognition of angry target-face expressions was selectively impaired by pleasant prime images which contained a face. For egocentric primes, recognition of neutral target-face expressions was significantly better than of angry expressions. Our results suggest that, first, the affective primacy hypothesis which predicts that affective information can be accessed automatically, preceding conscious cognition, holds true in subliminal priming only when the priming image contains a face. Second, egocentric primes interfere with the perception of angry target-face expressions suggesting that this vantage-point, directly relevant to the viewer, perhaps engages processes involved in action preparation which may weaken the priority of affect processing.

neuroscience↗