Huang, C. H. | Search bioRxiv

bioRxiv2025

WSInsight as a cloud-native pipeline for single-cell pathology inference on whole-slide images

WSInsight is an open-source platform for cohort-scale H&E whole-slide image analysis. It performs patch-level inference together with single-cell segmentation and phenotype classification, with morphology- and transcriptome-supervised cell-type heads retrainable from public data. Slides are read from cloud repositories and per-slide outputs are written to QuPath and OMERO. The same workflow is AI-agent callable through a Model Context Protocol endpoint. Applying it to TCGA cohorts recovered known immune and molecular associations.

bioinformatics↗

bioRxiv2022

The microtubular preprophase band recruits Myosin XI to the division site for plant cytokinesis

Plant growth is dependent on oriented cell divisions that employ the microtubular preprophase band (PPB) to position the cell plate. It has been intriguing how this transient cytoskeletal array imprints the spatial information to be read by the cytokinetic phragmoplast at later stages of mitotic cell division. In Arabidopsis thaliana, we discovered that the PPB recruited the Myosin XI motor MYA1 to the cortical division site where it joined microtubule-associated proteins and motors to form a ring of prominent cytoskeletal assemblies which received the expanding phragmoplast. This regulatory function of MYA1 in phragmoplast guidance is dependent on intact actin filaments. The discovery of these assemblies revealed the mechanism underlying how two dynamic cytoskeletal networks govern PPB-dependent division plane orientation during vegetative growth in flowering plants. ONE-SENTENCE SUMMARYMyosin XI joins microtubule-associated proteins and motors to form cortical assemblies to demarcate the cell division site.

plant biology↗