Biology subjects

Hoyos-Lopez, R.

Publications and source records attributed to Hoyos-Lopez, R..

3 recordsLinked to original sources

bioRxiv2018

Molecular Clock and Phylogeny of Anopheles species of the subgenus Nyssorhynchus (Diptera: Culicidae)

The main Phylogenetic hypothesis supporting the Myzorhynchella section as a natural group, but the sections Albimanus and Argyritarsis, do not present clearly resolved relationships, nor is it possible to recover the monophyly of both sections, even within these sections of Nyssorhynchus; it has not been possible identify the relationships between the species that make up these taxonomic subdivisions (Sallum, 2000, Sallum, 2002, Bourke, 2011, Foster, 2013). This lack of resolution has been attributed to the effect of few species for phylogenetic studies, making difficult the determination of monophyly of many groups, subgroups and complexes within sections Albimanus and Argyritarsis\n\nWe infer the phylogeny of the subgenus Nyssorhynchus through the sequences characterized for the molecular markers ND6, COI-Barcode, White and CAD, in addition we calculate the times of divergence for the main lineages corresponding to the sections Albimanus, Argyritarsis and Myzorhynchella using Bayesian approaches.

evolutionary biology↗

bioRxiv2018

Prediction of the secundary structure at the tRNASer (UCN) of Lutzomyia longipalpis (Diptera: Psychodidae)

Lutzomyia longipalpis is the main vector of Leishmania infantum, the etiological agent of visceral leishmaniasis in America and Colombia. Taxonomically belongs to the subgenus Lutzomyia, which includes other vector species that exhibit high morphological similarity to the female species difficult to identify vectors in leishmaniasis foci and suggesting the search for molecular markers that facilitate this task, further researchs with mitochondrial genes, chromosome banding, reproductive isolation and pheromones evidence the existence of species complex. The aim of this study was to predict the secondary structure of mitochondrial transfer RNA serine (tRNASer) for UCN codon of Lutzomyia longipalpis as molecular marker for identify of this species. Sequences recorded in Genbank of L. longipalpis sequences were aligned with tRNA's from previously described species and then tRNASer secondary structure was inferred by software tRNAscan-SE 1.21. The length of tRNASer was 67 base pairs (bp). Two haplotypes were detected in the five sequences analyzed. The L. longipalpis tRNASer showed 7 intrachain pairing in the acceptor arm, 3 in the DHU arm, 4 in the anticodon arm and 5 in the T{psi}C. The size of the loops corresponded to 5 nucleotides in the DHU, 7 in the anticodon, 4 in the variable and 7 in the T{psi}C. L. longipalpis is distinguished from other species at subgenera Lutzomyia by the secondary structure and substitutions inferred tRNASer evidenced in the primary sequence.

molecular biology↗

bioRxiv2018

Molecular Typing with COI - DNA Barcode of mosquitoes with medical importance from rural area from La Pintada, Antioquia, Colombia

DNA barcode is a methodology that allows the identification of species using a short fragment of cytochrome oxidase I and library sequences stored in the barcode of life database (bold>), make up an alternative tool for mosquito identification in areas epidemiologically active for arboviruses, protozoa and bacteria. In our study, we collected 114 adult mosquitoes in a rural area in the municipality of La Pintada (Antioquia, Colombia), and were separate for genus and species using morphological keys. Two Legs were taken of specimens mounted, and these were used for DNA extraction, amplification of COI-Barcode through PCR and sequencing. 38 sequences were characterized of seven mosquito species and used in bold> for molecular identification, subsequent characterization of genetic distances intra/interspecies, and MOTUs grouping by neighbor-joining analyses. Seven MOTUs were separate corresponding to seven species identify by morphological keys. bold> was able to identify five species, and two were identified to the genre. The following medically important mosquitoes were recorded in the rural area from La Pintada (Antioquia): Aedes aegypti, Anopheles triannulatus, Coquillettidia nigricans, Mansonia titillans, Ochlerotatus angustivitatus, Psorophora ferox and Psorophora (Grabhamia) sp.

zoology↗