Biology subjects

Holt, N. C.

Publications and source records attributed to Holt, N. C..

3 recordsLinked to original sources

bioRxiv2024

Does force depression resulting from shortening against series elasticity contribute to the activation dependence of optimum length?

The optimum length for force generation (L0) increases as activation is reduced, challenging classic theories of muscle contraction. Although the activation dependence of L0 is seemingly consistent with length-dependent Ca2+ sensitivity, this mechanism cant explain the apparent force dependence of L0, or the effect of series compliance on activation-related shifts in L0. We have tested a theory proposing that the activation dependence of L0 relates to force depression resulting from shortening against series elasticity. This theory predicts that significant series compliance would cause tetanic L0 to be shorter than the length corresponding to optimal filament overlap, thereby increasing the activation dependence of L0. We tested this prediction by determining L0 and maximum tetanic force (P0) with (L0_spring, P0_spring) and without added compliance in bullfrog semitendinosus muscles. The activation dependence of L0 was characterised with the addition of twitch and doublet contractions. Springs attached to muscles gave added fixed-end compliances of 11-39%, and this added compliance induced force depression for tetanic fixed-end contractions (P0_spring/P0 < 1). We found strong, negative correlations between spring compliance and both P0_spring (r2 = 0.89-91) and L0_spring (r2 = 0.60-63; P < 0.001), while the activation dependence of L0 was positively correlated to added compliance (r2 = 0.45, P = 0.011). However, since the compliance-mediated reduction in L0 was modest relative to the activation-related shift reported for the bullfrog plantaris muscle, additional factors must be considered. Our demonstration of force depression under novel conditions adds support to the involvement of a stress-induced inhibition of cross-bridge binding.

physiology↗

bioRxiv2024

Beyond power limits: the kinetic energy capacity of skeletal muscle

Muscle is the universal agent of animal movement, and limits to muscle performance are therefore an integral aspect of animal behaviour, ecology, and evolution. A mechanical perspective on movement makes it amenable to analysis from first principles, and so brings the seeming certitude of simple physical laws to the challenging comparative study of complex biological systems. Early contributions on movement biomechanics considered muscle energy output to be limited by muscle work capacity, Wmax; triggered by seminal work in the late 1960s, it is now held broadly that a complete analysis of muscle energy output is to also consider muscle power capacity, for no unit of work can be delivered in arbitrarily brief time. Here, we adopt a critical stance towards this paradigmatic notion of a power-limit, and argue that the alternative constraint to muscle energy output is instead imposed by a characteristic kinetic energy capacity, Kmax, dictated by the maximum speed with which the actuating muscle can shorten. The two critical energies can now be directly compared, and define the physiological similarity index, {Gamma} = Kmax/Wmax. It is the explanatory power of this comparison that lends weight to a shift in perspective from muscle power to kinetic energy capacity, as is argued through a series of brief illustrative examples. {Gamma} emerges as an important dimensionless number in musculoskeletal dynamics, and sparks novel hypotheses on functional adaptations in musculoskeletal "design" that depart from the parsimonious evolutionary null hypothesis of geometric similarity.

zoology↗

bioRxiv2019

Tuning of feedforward control enables stable muscle force length dynamics after loss of autogenic proprioceptive feedback

Animals must integrate feedforward, feedback and intrinsic mechanical control mechanisms to maintain stable locomotion. Recent studies of guinea fowl (Numida meleagris) revealed that the distal leg muscles rapidly modulate force and work output to minimize perturbations in uneven terrain. Here we probe the role of reflexes in the rapid perturbation response of muscle by studying the effects of proprioceptive loss. We induced bilateral loss of autogenic proprioception in the lateral gastrocnemius muscle (LG) using self-reinnervation. We compared ankle kinematics and in vivo muscle dynamics in birds with reinnervated LG and intact LG. Reinnervated and intact muscles exhibit similar force-length dynamics, with rapid changes in work to stabilize running obstacle terrain. Reinnervated LG exhibits 23ms earlier steady-state activation, consistent with feedforward tuning of activation phase to compensate for lost proprioception. Modulation of force duration is impaired in rLG, confirming the role of reflex feedback in regulating force duration in intact muscle.

physiology↗