Hoinka, J. | Search bioRxiv

bioRxiv2018

Novel analysis of HT-SELEX data elucidates the role of DNA shape in transcription factor binding

Understanding the principles of DNA binding by transcription factors (TFs) is of primary importance for studying gene regulation. Recently, several lines of evidence suggested that both DNA sequence and shape contribute to TF binding. However, the question if in the absence of any sequence similarity to the binding motif, DNA shape can still increase probability of binding was yet to be addressed.\n\nTo address this challenge, we developed Co-SELECT, a computational approach to analyze the results of in vitro HT-SELEX experiments for TF-DNA binding. Specifically, the presence of motif-free sequences in late HT-SELEX rounds and their enrichment in weak binders allowed us to detect evidence for the role of DNA shape features in TF binding.\n\nOur approach revealed that, even in the absence of the sequence motif, TFs have propensity to weakly bind to DNA molecules enriched in specific shape features. Surprisingly, we also found that some properties of DNA shape contribute to promiscuous binding of all tested TF families. Strikingly, such promiscuously bound shapes correspond to the most frequent shape formed by the DNA. We propose that this promiscuous binding facilitates diffusing of TFs along the DNA molecule before it is locked in its binding site.

bioinformatics↗

bioRxiv2017

AptaBlocks: Accelerating the Design of RNA-based Drug Delivery Systems

Synthetic RNA molecules are increasingly used to alter cellular functions. These successful applications indicate that RNA-based therapeutics might be able to target currently undruggable genes. However, to achieve this promise, an effective method for delivering therapeutic RNAs into specific cells is required. Recently, RNA aptamers emerged as promising delivery agents due to their ability of binding specific cell receptors. Crucially, these aptamers can frequently be internalized into the cells expressing these receptors on their surfaces. This property is leveraged in aptamer based drug delivery systems by combining such receptor-specific aptamers with a therapeutic \"cargo\" such that the aptamer facilitates the internalization of the cargo into the cell. The advancement of this technology however is contingent on an efficient method to produce stable molecular complexes that include specific aptamers and cargoes. A recently proposed experimental procedure for obtaining such complexes relies on conjugating the aptamer and the cargo with complementary RNA strands so that when such modified molecules are incubated together, the complementary RNA strands hybridize to form a double-stranded \"sticky bridge\" connecting the aptamer with its cargo. However, designing appropriate sticky bridge sequences guaranteeing the formation and stability of the complex while simultaneously not interfering with the aptamer or the cargo as well as not causing spurious aggregation of the molecules during incubation has proven highly challenging. To fill this gap, we developed AptaBlocks, a computational method to design sticky bridges to connect RNA-based molecules (blocks). AptaBlocks relies on a biophysically inspired theoretical model capturing the complex objectives of the design and yet is simple enough to allow for efficient parameter estimation. Given this model, the sticky bridge sequence is optimized using a Monte Carlo algorithm based on heat-bath transitions. The effectiveness of the algorithm has been verified computationally and experimentally. AptaBlocks can be used in variety of experimental settings and its preliminary version has already been leveraged to design an aptamer based delivery system for a cytotoxic drug targeting Pancreatic ductal adenocarcinoma cells. It is thus expected that AptaBlocks will play a substantial role in accelerating RNA-based drug delivery design.\n\nAptaBlocks is available at https://github.com/wyjhxq/AptaBlocks.

bioinformatics↗