Biology subjects

Ho, Y.-S.

Publications and source records attributed to Ho, Y.-S..

3 recordsLinked to original sources

bioRxiv2023

The Role of PKR on Modulating Neuroinflammation and Cognitive Dysfunctions in a Peripheral Inflammation Model

Systemic inflammation may promote neuroinflammation and neurodegeneration. The double-stranded RNA-dependent protein kinase (PKR) is a key signaling molecule that regulates immune responses. This study aims to examine the role of PKR in regulating systemic inflammation-induced neuroinflammation and cognitive dysfunctions using a laparotomy mouse model. In the first part, wild-type C57BL/6J and C57BL/6-Tg(CD68-EGFP)1Drg/J mice were assigned to undergo either laparotomy with sevoflurane anesthesia or sevoflurane alone to examine effects of systemic inflammation on neuroinflammation and cognition. In the second part, PKR-/- mice were used to study the role of PKR in modulating laparotomy-induced systemic inflammation, neuroinflammation, and cognition. For the third part, PKR was inhibited selectively in cholinergic neurons of ChAT-IRES-Cre-eGFP mice via intracerebroventricular injection of rAAV-DIO-PKR-K296R. This examined the effects of inhibiting PKR in cholinergic neurons on glucose metabolism and cognition in the laparotomy model. Our study revealed that genetic deletion of PKR in mice potently attenuated the laparotomy-induced peripheral and neural inflammation and cognitive deficits. Furthermore, inhibiting PKR in the cholinergic neurons rescued the laparotomy-induced brain glucose hypometabolism and cognitive impairment. Our results demonstrated the critical role of PKR in regulating neuroinflammation and cognitive dysfunctions in a peripheral inflammation model. PKR could be a pharmacological target for treating systemic inflammation-induced neuroinflammation and cognitive dysfunctions.

neuroscience↗

bioRxiv2020

Classic articles in Apoptotic Research: A Bibliometric Analysis

BackgroundClassic articles are defined as research papers with a total citation of one thousand or more. The present study is to identify and analyse the characteristics of the classic articles in apoptosis research. MethodClassic articles with total of 1,000 or more citations from Web of Science Core Collection since year of publication to the end of 2017 were basically assessed regarding their document types, languages, journals, and Web of Science categories within 1900 to 2017. ResultThe study showed 418 classic documents in apoptotic research including 260 articles published between 1972 and 2012. The most productive Web of Science category was multidisciplinary sciences. Nature published most of these classic articles followed by Cell, and Science. The most productive country and institution were United States and Harvard University respectively. The author S.J. Korsmeyer from Harvard University was the most productive in apoptosis field and published 13 classic apoptosis articles while the author J.C Reed had more potential to publish classic apoptosis articles in future. The author J.C. Reed and V.A. Fadok had equal potential to publish the same number of classic articles as first- and corresponding-author. Article of Kerr et al. in 1972 was the most popular and cited apoptosis article. The most impact article in 2017 was article entitled "Tumor-associated B7-H1 promotes T-cell apoptosis: A potential mechanism of immune evasion" by Dong et al. in 2002. ConclusionThis study seems to identify the most industrious authors, institutions, and countries in the field of apoptosis research. It also tends to reveal the historical and discoveries related to the pathophysiology of apoptosis as well as the most impact publications on apoptosis studies.

cell biology↗

bioRxiv2018

Matrin 3-dependent neurotoxicity is modified by nucleic acid binding and nucleocytoplasmic localization

Abnormalities in nucleic acid processing are associated with the development of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Mutations in Matrin 3 (MATR3), a poorly understood DNA- and RNA-binding protein, cause familial ALS/FTD, and MATR3 pathology is a feature of sporadic disease, suggesting that MATR3 dysfunction is integrally linked to ALS pathogenesis. Using a primary neuron model to assess MATR3-mediated toxicity, we noted that neurons were bidirectionally vulnerable to MATR3 levels, with pathogenic MATR3 mutants displaying enhanced toxicity. MATR3s zinc finger domains partially modulated toxicity, but elimination of its RNA recognition motifs had no effect on neuronal survival, instead facilitating its self-assembly into liquid-like droplets. In contrast to other RNA-binding proteins associated with ALS, cytoplasmic MATR3 redistribution mitigated neurodegeneration, suggesting that nuclear MATR3 mediates toxicity. Our findings offer a foundation for understanding MATR3-related neurodegeneration and how nucleic acid binding functions, localization, and pathogenic mutations drive sporadic and familial disease.

cell biology↗