A Biologically Informed Heterogeneous Graph Neural Network for Multi-Task Prediction of ncRNA-Metastasis-Cancer Interactions
Metastasis involves context-dependent molecular interactions in which non-coding RNAs, particularly miRNAs and circRNAs, play important regulatory roles. However, existing computational approaches generally do not jointly represent cancer type, metastatic event, and cancer-specific metastatic context. We developed a context-aware multi-task heterogeneous graph neural network (GNN) for predicting ncRNA associations with cancer types and metastatic events. The framework integrates multiple biological repositories into a heterogeneous graph representing ncRNAs, cancers, metastatic event types (METs), and cancer-specific metastatic instances (CSMIs). The model performs six link-prediction tasks using a hierarchical transformer-based encoder and multi-relational TuckER decoder. Across ten independently initialized runs evaluated on the RNA-group-disjoint held-out test set, the model achieved a global AUROC of 0.8801 {+/-} 0.0118 and an F1 score of 0.8260 {+/-} 0.0071. All three ablation variants yielded lower AUROC, with the largest reduction under independent task training. Case studies in pancreatic cancer, colorectal cancer, and hepatocellular carcinoma provided disease-level, event-level, and expression-based support, respectively, for top-ranked candidate associations. The framework enables context-specific prioritization of ncRNA-cancer-metastasis associations for experimental evaluation.