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Biology subjects

Hart, P. J.

Publications and source records attributed to Hart, P. J..

3 recordsLinked to original sources

Calibrating Classifiers Across Covariates: Hierarchical Vocal Density Estimation for Acoustic Monitoring at Scale

Passive acoustic sensors and machine learning classifiers offer a scalable approach to monitoring wildlife populations. These classifiers typically detect whether a species is present in a short time-window of audio; ecologists, however, want ecologically meaningful measures such as indices of site-level abundance. Vocal density, the proportion of time-windows containing a vocalization, is a useful abundance proxy. Recovering vocal density from classifiers requires calibrating their outputs against expert-labeled data. Because classifier performance varies between sites, a single global calibration yields overconfident site-level estimates, yet calibrating every site independently requires prohibitive labeling effort. Estimating site-level vocal density at the scale of modern sensor networks demands a label-efficient alternative. We present a Bayesian hierarchical extension of Platt scaling, a commonly used calibration method, that calibrates classifiers at the site level while borrowing strength across sites. Where site-level covariates are available, a Gaussian-process prior lets the model learn how calibration varies across covariate space. Averaging calibrated per-clip probabilities across a site's recordings yields a posterior distribution over vocal density, and we show how to carry that uncertainty into downstream regressions of vocal density on environmental covariates. Across simulations and two fully annotated field datasets from Hawai'i and the Pacific Northwest, our model attained desired credible-interval coverage of vocal density estimates with narrower intervals than independent site-level calibration at equal labeling effort, and lower mean squared error. Global calibration, by contrast, failed to attain coverage except when simulated sites were genuinely homogeneous. Unlike site-level calibration, our model enables calibration at sites with zero labeled data, and incorporating covariates further improved precision when site heterogeneity was covariate-driven. Applied to a 283-site dataset in Pennsylvania with only two labeled clips per site, we recovered known habitat associations for the declining Wood Thrush (Hylocichla mustelina). Our method provides a principled, label-efficient route from bioacoustic classifier scores to site-level abundance indices with well-quantified uncertainty, making rigorous ecological inference feasible at the scale at which acoustic sensor networks are now deployed.

ecology↗

Acoustic niche partitioning in two tropical wet forest bird communities

When acoustic signals sent from individuals overlap in frequency and time, acoustic interference and signal masking may occur. Under the acoustic niche hypothesis (ANH), signaling behavior has evolved to minimize overlap with other calling individuals through selection on signal structure and the senders ability to adjust the timing of signals. In this study, we examine the fine-scale use of acoustic space and the relevance of the acoustic niche hypothesis in two montane tropical wet forest bird communities (Costa Rica and Hawaii) that vary in bird species richness. We used a null model approach to test the prediction that there are differences between observed and expected signal overlap in both communities. As predicted under ANH, we found much lower overlap of acoustic signals than expected by chance. In addition, spectral and temporal overlap between different signals was far more common in Hawaii than Costa Rica. These findings constitute strong support that there is competition for acoustic space in signaling communities, and this has resulted in temporal and spectral partitioning of the soundscape.

animal behavior and cognition↗

Oxamniquine resistance alleles are widespread in Old World Schistosoma mansoni and predate drug deployment

Do mutations required for adaptation occur de novo, or are they segregating within populations as standing genetic variation? This question is key to understanding adaptive change in nature, and has important practical consequences for the evolution of drug resistance. We provide evidence that alleles conferring resistance to oxamniquine (OXA), an antischistosomal drug, are widespread in natural parasite populations under minimal drug pressure and predate OXA deployment. OXA has been used since the 1970s to treat Schistosoma mansoni infections in the New World where S. mansoni established during the slave trade. Recessive loss-of-function mutations within a parasite sulfotransferase (SmSULT-OR) underlie resistance, and several verified resistance mutations, including a deletion (p.E142del), have been identified in the New World. Here we investigate sequence variation in SmSULT-OR in S. mansoni from the Old World, where OXA has seen minimal usage. We sequenced exomes of 204 S. mansoni parasites from West Africa, East Africa and the Middle East, and scored variants in SmSULT-OR and flanking regions. We identified 39 non-synonymous SNPs, 4 deletions, 1 duplication and 1 premature stop codon in the SmSULT-OR coding sequence, including one confirmed resistance deletion (p.E142del). We expressed recombinant proteins and used an in vitro OXA activation assay to functionally validate the OXA-resistance phenotype for four predicted OXA-resistance mutations. Three aspects of the data are of particular interest: (i) segregating OXA-resistance alleles are widespread in Old World populations (4.29 - 14.91% frequency), despite minimal OXA usage, (ii) two OXA-resistance mutations (p.W120R, p.N171IfsX28) are particularly common (>5%) in East African and Middle-Eastern populations, (iii) the p.E142del allele has identical flanking SNPs in both West Africa and Puerto Rico, suggesting that parasites bearing this allele colonized the New World during the slave trade and therefore predate OXA deployment. We conclude that standing variation for OXA resistance is widespread in S. mansoni.\n\nAUTHOR SUMMARYIt has been argued that drug resistance is unlikely to spread rapidly in helminth parasites infecting humans. This is based, at least in part, on the premise that resistance mutations are rare or absent within populations prior to treatment, and take a long time to reach appreciable frequencies because helminth parasite generation time is long. This argument is critically dependent on the starting frequency of resistance alleles - if high levels of \"standing variation\" for resistance are present prior to deployment of treatment, resistance may spread rapidly. We examined frequencies of oxamniquine resistance alleles present in Schistosoma mansoni from Africa and the Middle East where oxamniquine has seen minimal use. We found that oxamniquine resistance alleles are widespread in the Old World, ranging from 4.29% in the Middle East to 14.91% in East African parasite populations. Furthermore, we show that resistance alleles from West African and the Caribbean schistosomes share a common origin, suggesting that these alleles travelled to the New World with S. mansoni during the transatlantic slave trade. Together, these results demonstrate extensive standing variation for oxamniquine resistance. Our results have important implications for both drug treatment policies and drug development efforts, and demonstrate the power of molecular surveillance approaches for guiding helminth control.

evolutionary biology↗