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Biology subjects

Guasto, J. S.

Publications and source records attributed to Guasto, J. S..

5 recordsLinked to original sources

Early viral infection of cyanobacteria drives bacterial chemotaxis in the oceans

Interactions among marine microbes primarily occur through exudation and sensing of dissolved chemical compounds, which ultimately control ecosystem-scale processes such as biomass production, nutrient cycling, carbon fixation, and remineralization. Prior to lysis, viruses alter host metabolism, stimulating the release of dissolved chemical cues from intact plankton. However, the nature and degree of interactions between prelysis, virus-infected cells and neighbouring microbes remain unquantified. Here, we determine the impact of viral infection on dissolved metabolite pools from the marine cyanobacterium Synechococcus and the subsequent chemotactic response of heterotrophic bacteria using time-resolved metabolomics and microfluidics. Metabolites released from intact, virus-infected Synechococcus elicited vigorous chemoattractive responses from heterotrophic bacteria (Vibrio alginolyticus and Pseudoalteromonas haloplanktis), with the strongest responses occurring in the early infection stages and following cell lysis. We provide the first experimental observations of sustained chemotaxis towards live, infected Synechococcus, which is contrasted by no discernible chemotaxis toward uninfected Synechococcus. Finally, metabolite compounds and concentrations driving chemotactic responses were identified using a novel high-throughput microfluidic device. Our findings establish that prior to cell lysis, virus-infected picophytoplankton release compounds that significantly attract motile heterotrophic bacteria, illustrating a viable mechanism for resource transfer to chemotactic bacteria with implications for our understanding of carbon and nutrient flux across trophic levels.

biophysics↗

Multiplexed microfluidic screening of bacterial chemotaxis

Microorganism sensing of and responding to ambient chemical gradients regulates a myriad of microbial processes that are fundamental to ecosystem function and human health and disease. The development of efficient, high-throughput screening tools for microbial chemotaxis is essential to disentangling the roles of diverse chemical compounds and concentrations that control cell nutrient uptake, chemorepulsion from toxins, and microbial pathogenesis. Here, we present a novel microfluidic multiplexed chemotaxis device (MCD) which uses serial dilution to simultaneously perform six parallel bacterial chemotaxis assays that span five orders of magnitude in chemostimulant concentration on a single chip. We first validated the dilution and gradient generation performance of the MCD, and then compared the measured chemotactic response of an established bacterial chemotaxis system (Vibrio alginolyticus) to a standard microfluidic assay. Next, the MCDs versatility was assessed by quantifying the chemotactic responses of different bacteria (Psuedoalteromonas haloplanktis, Escherichia coli) to different chemoattractants and chemorepellents. The MCD vastly accelerates the chemotactic screening process, which is critical to deciphering the complex sea of chemical stimuli underlying microbial responses.

ecology↗

Dynamic mode structure of active turbulence

Dense suspensions of swimming bacteria exhibit chaotic flow patterns that promote the mixing and transport of resources and signalling chemicals within cell colonies. While the importance of active turbulence is widely recognized, the structure and dynamics of the resulting collective flows are the subject of intense investigation. Here, we combine microfluidic experiments with proper orthogonal decomposition (POD) analysis to quantify the dynamical flow structure of this model active matter system under a variety of conditions. In isotropic bulk turbulence, the modal representation shows that the most energetic flow structures dictate the spatio-temporal dynamics across a range of suspension activity levels. In confined geometries, POD analysis illustrates the role of boundary interactions for the transition to bacterial turbulence, and it quantifies the evolution of coherent active structures in externally applied flows. Beyond establishing the physical flow structures under-pinning the complex dynamics of bacterial turbulence, the low-dimensional representation afforded by this modal analysis offers a potential path toward data-driven modelling of active turbulence.

biophysics↗

Viscophobic turning regulates microalgae transport in viscosity gradients

Gradients in fluid viscosity characterize microbiomes ranging from mucus layers on marine organisms1 and human viscera2,3 to biofilms4. While such environments are widely recognized for their protective effects against pathogens and their ability to influence cell motility2,5, the physical mechanisms regulating cell transport in viscosity gradients remain elusive6-8, primarily due to a lack of quantitative observations. Through microfluidic experiments, we directly observe the transport of model biflagellated microalgae (Chlamydomonas reinhardtii) in controlled viscosity gradients. We show that despite their locally reduced swimming speed, the expected cell accumulation in the viscous region9,10 is stifled by a viscophobic turning motility. This deterministic cell rotation - consistent with a flagellar thrust imbalance11,12 - reorients the swimmers down the gradient, causing their accumulation in the low viscosity zones for sufficiently strong gradients. Corroborated by Langevin simulations and a three-point force model of cell propulsion, our results illustrate how the competition between viscophobic turning and viscous slowdown ultimately dictates the fate of population scale microbial transport in viscosity gradients.

biophysics↗

Ingestible Osmotic Pill for In-vivo Sampling of Gut Microbiome

Technologies capable of non-invasively sampling different locations in the gut upstream of the colon will enable new insights into the role of organ-specific microbiota in human health. We present an ingestible, biocompatible, battery-less, 3D-printed micro-engineered pill with integrated osmotic sampler and microfluidic channels for in vivo sampling of the gut lumen and its microbiome upstream of the colon. The pills sampling performance was characterized using realistic in vitro models and validated in vivo in pigs and primates. Our results show that the bacterial populations recovered from the pills microfluidic channels closely resemble the bacterial population demographics of the microenvironment to which the pill was exposed. We believe such lab-on-a-pill devices will revolutionize our understanding of the spatial diversity of the gut microbiome and its response to medical conditions and treatments.

bioengineering↗