Biology subjects

Grytsay, A.

Publications and source records attributed to Grytsay, A..

3 recordsLinked to original sources

bioRxiv2025

Allelic Variation in CYP3A4 and PLB1 Drives Feed Efficiency and Immunometabolic Resilience in Beef Cattle

We evaluated genetic markers for feed efficiency and immunocompetence in 108 crossbred steers (217 {+/-} 8.2 kg) fed a high-forage total mixed ration for 35 days, using GrowSafe8000 intake nodes to calculate residual feed intake (RFI). From the 20 most efficient (low-RFI) and 20 least efficient (high-RFI) animals, we genotyped three metabolic loci (CYP3A4 rs438103222, PLB1 rs456635825, CRAT rs876019788) and profiled blood mRNA levels of these plus eight innate/adaptive immune genes. Logistic regression revealed that CYP3A4 and PLB1 polymorphisms--but not CRAT--were strongly associated with initial and final body weight, average daily gain, and feed intake: CYP3A4 A/A and PLB1 A-allele carriers achieved superior growth on reduced feed. Haplotype reconstruction across the three loci defined eight multi-SNP combinations, with the C-A-A haplotype enriched in low-RFI steers and combinations harboring CYP3A4 A and PLB1 A alleles linked to low RFI. Intriguingly, these favorable genotypes also overlapped with up-regulation of immune sensors and effectors (e.g., CD14, TLR4, TNF-), indicating a coordinated metabolic-immune adaptation in efficient cattle. Collectively, our results validate CYP3A4 and PLB1 as high-impact quantitative trait nucleotides for marker-assisted selection aimed at simultaneously improving feed efficiency and immune resilience in beef production.

genetics↗

bioRxiv2022

Molecular evolution and inheritance pattern of Sox gene family among Bovidae

Sox gene is an evolutionarily conserved family of transcription factors that play important roles in cellular differentiation and numerous complex developmental processes. In vertebrates, Sox proteins are required for cell fate decision, morphogenesis, and control self-renewal in embryonic and adult stem cells. Sox gene family has been well studied in multiple species including humans but scanty or no study in Bovidae. In this study, we conducted a detailed evolutionary analysis of this gene family in Bovidae, including their physicochemical properties, biological functions, and patterns of inheritance. We performed a genome-wide cataloguing to explore the Sox gene family using multiple bioinformatics tools. Our analysis revealed conserved motifs that are crucial to the ability of Sox genes to interact with the regulatory regions of target genes and orchestrate multiple developmental and physiological processes. Importantly, we report a unique motif being EFDQYL/ELDQYL found in SoxE and SoxF groups. Further analysis revealed that this motif sequence accounts for the binding and transactivation potential of Sox proteins. Protein-protein interaction showed significant interaction among Sox genes and related genes implicated in embryonic development and the regulation of cell differentiation. We conclude that Sox gene family uniquely evolved among Bovidae with a few exhibiting important motifs that drives several developmental and physiological processes.

evolutionary biology↗

bioRxiv2022

Deciphering inhibitory mechanism of coronavirus replication through host miRNAs-RNA-dependent RNA polymerase (RdRp) interactome

Despite what we know so far, Covid-19, caused by SARS-CoV-2 virus, remains a pandemic that still require urgent healthcare intervention. The frequent mutations of the SARS-CoV-2 virus has rendered disease control with vaccines and antiviral drugs quite difficult and challenging, with newer variants surfacing constantly. There is therefore the need for newer, effective and efficacious drugs against coronaviruses. Considering the role of RNA dependent, RNA polymerase (RdRp) as an important enzyme necessary for the virus life cycle and its conservation among coronaviruses, we investigated potential host miRNAs that can be employed as broad-range antiviral drugs averse to coronaviruses, with particular emphasis on BCoV, MERS-CoV, SARS-CoV and SARS-CoV-2. miRNAs are small molecules capable of binding mRNA and regulate expression at transcriptional or translational levels. Our hypothesis is that host miRNAs have the potential of blocking coronavirus replication through miRNA-RdRp mRNA interaction. To investigate this, we downloaded the open reading frame (ORF 1ab) nucleotide sequences and used them to interrogate miRNA databases for miRNAs that can bind them. We employed various bioinformatics tools to predict and identify the most effective host miRNAs. In all, we found 27 miRNAs that target RdRp mRNA of multiple coronaviruses, of which three - hsa-miR-1283, hsa-miR-579-3p, and hsa-miR-664b-3p target BCoV, SARS-CoV and SARS-CoV-2. Additionally, hsa-miR-374a-5p has three bovine miRNAs homologs viz bta-miR-374a, bta-miR-374b, and bta-miR-374c. Inhibiting the expression of RdRp enzyme via non-coding RNA is novel and of great therapeutic importance in the control of coronavirus replication, and could serve as a broad-spectrum antiviral, with hsa-miR-1283, hsa-miR-579-3p, and hsa-miR-664b-3p highly promising.

genomics↗