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Biology subjects

Grenville-Briggs, L. J.

Publications and source records attributed to Grenville-Briggs, L. J..

4 recordsLinked to original sources

Plant LETM1 homologs are required for fungus-induced antibiotic resistance and biostimulation

Our findings confirm that CIRA15A / LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN1 (LETM1) is critical for Trichoderma-induced growth biostimulation in sugar beet and the Cellulase-Induced Resistance to Alamethicin (CIRA) response in Arabidopsis. Notably, this plant homolog of a gene associated with human disease plays a vital role in both defense against Trichoderma antimicrobial peptides and the biostimulation of plant growth. We identified AtCIRA15A/LETM1 and AtCIRA15B/LETM2 as key genetic determinants of CIRA through Arabidopsis analysis and comparative studies of sugar beet inbred lines. BvLETM1 allelic variations correlated with differential biostimulation responses, and complementation confirmed functional LETM1 alleles restore CIRA in Arabidopsis mutants. These findings highlight LETM1 as a crucial factor in Trichoderma-plant interactions, with potential applications in breeding for enhanced microbial-induced plant biostimulation and agricultural productivity.

plant biology↗

Comparative high-throughput phenotyping across two facilities reveals differential impact of defence mechanisms on plant growth and development.

Fitness costs of plant disease defence are often subtle and difficult to quantify. In this study, we therefore used comparative high-throughput phenotyping in two independent facilities to assess growth, morphology and physiology of potato (cv. Desiree) with high time-resolution monitoring different defence mechanisms under pathogen-free conditions. Plants were either treated weekly with the resistance inducers {beta}-aminobutyric acid (BABA; 10 mM) or potassium phosphite (KPhi; 36 mM) or comprised six transgenic lines expressing late blight resistance genes (single Rpi genes or a three-gene stack) or reduced jasmonate perception (StCOI1-RNAi). Over four weeks, image-derived traits revealed consistent cross-facility effects for plant height and colour: BABA treatment increased plant height but reduced canopy area and induced a paler greenness signature, whereas KPhi caused minimal and transient growth effects. Chlorophyll fluorescence at the NaPPI facility indicated reduced vitality (Rfd_Lss) in BABA-treated plants and increased Rfd_Lss following KPhi, while maximum PSII efficiency was largely unchanged. Several transgenic lines showed somewhat reduced above-ground biomass. Enzyme activity profiling produced distinct treatment and genotype signatures, but was strongly modulated by facility conditions that overrode these specificities. Overall, high-throughput phenotyping robustly detected subtle growth-defence trade-offs across platforms. HighlightHigh-throughput optical phenotyping validated across two independent research facilities reveals that stacked resistance genes and resistance inducers in potato trigger subtle growth trade-offs. Graphical abstracts O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=97 SRC="FIGDIR/small/713143v1_ufig1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@89df47org.highwire.dtl.DTLVardef@1a1ce64org.highwire.dtl.DTLVardef@1f52f0dorg.highwire.dtl.DTLVardef@1e41c35_HPS_FORMAT_FIGEXP M_FIG C_FIG Experimental timeline for high-throughput plant phenotyping platforms. Created in BioRender. Poque, S. (2026) https://BioRender.com/nmkve7g

plant biology↗

Identifying gaps between scientific and local knowledge in climate change adaptation for northern European agriculture

Climate change impacts agriculture in complex and regionally specific ways. In temperate regions such as southern Sweden, it presents both opportunities, such as longer growing seasons, and challenges, including increased drought and heat stress. This study examined how Swedish agriculture is adapting to climate change, using the extreme 2018 drought and heatwave as a critical case to explore both immediate responses and longer-term preparedness in crop production systems. We compared scientific and local knowledge through a systematic scoping review of peer-reviewed literature from northern Europe, a workshop with project scientists, and a participatory workshop with farmers. The study aimed to identify gaps between scientific and local understandings of climate change adaptation, particularly in how extreme events influence decision-making and preparedness. Using a transdisciplinary, problem-feeding approach, we compared how farmers and scientists conceptualise crisis response and long-term adaptation, employing influence diagrams to visualise their understandings. Our review found that much scientific work focuses on short-term coping strategies, such as feed substitution or altered irrigation, while longer-term adaptation remains underexplored. In contrast, farmers emphasized context specific actions grounded in their farm operations, highlighting socio-economic and administrative constraints, whereas scientists tended to propose generalisable technology-oriented strategies. These differences underscore the importance of integrating both experiential and scientific knowledge to co-develop more effective adaptation responses. The study lays the foundation for a targeted, large-scale survey to explore farmer motivations, perceptions of risk and opportunity, and adaptive capacities across diverse contexts. Our findings highlight the need to move beyond reactive measures and investigate anticipatory strategies that foster and enable adaptive capacity. Here we demonstrate that supporting long-term adaptation in agriculture will require inclusive, evidence-based approaches that incorporate perspectives from farmers, advisors, scientists, and public authorities alike.

scientific communication and education↗

Double trouble or a blessing in disguise? Co-infection of potato with the causal agents of late and early blight

The simultaneous occurrence of multiple diseases is an understudied area in plant pathology; however, studies of animal and human diseases have shown that the presence of multiple pathogens can impact virulence, and the course of disease development. Furthermore, they also present an important driver of epidemiological dynamics. Global potato production is plagued by multiple pathogens, amongst which are Phytophthora infestans and Alternaria solani, the causal agents of potato late and early blight respectively. Both these pathogens have different lifestyles and are successful pathogens of potato, but despite observations of both pathogens infecting potato simultaneously in field conditions, the tripartite interactions between potato and these two pathogens are so far, poorly understood. Here we studied the interaction of A. solani and P. infestans first in vitro and subsequently in planta both in laboratory and field settings. We found that A. solani can inhibit P. infestans both in terms of growth in vitro and infection of potato, both in laboratory experiments and in an agriculturally relevant field setting.

microbiology↗