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Gonzalez-Tobon, J.

Publications and source records attributed to Gonzalez-Tobon, J..

3 recordsLinked to original sources

Microscopical and molecular characterization of the infection cycle of Phytophthora betacei during disease development on tree tomato (Solanum betaceum).

Phytophthora betacei is a recently described oomycete plant pathogen closely related to Phytophthora infestans sensu stricto. This plant pathogen naturally infects tree tomato (Solanum betaceum) but has not been reported on tomatoes and potatoes, the primary hosts of P. infestans. The aim of this study was to characterize the infection cycle of P. betacei using microscopy and molecular approaches. Several strains were inoculated in susceptible tree tomato plants and disease progression was monitored via six epidemiological parameters. Although different P. betacei strains displayed a highly variable disease phenotype, the most aggressive one was chosen for further plant inoculations. Samples at different time points of the infection cycle were analyzed at the cellular level via light and scanning electron microscopy (SEM) and at the molecular level via qRT-PCR of infection-stage-specific markers. The infection cycle of P. betacei differed from that of P. infestans in having a longer biotrophic stage, larger lesions, and higher sporulation capacity. Additionally, P. betacei transcriptomic profiles were monitored along the infection cycle via RNAseq and evidenced a changing expression landscape that supports an elongated hemibiotrophic transition and a clear distinction from what is being expressed in the mycelium or the sporangia. This study provides novel insights into the interaction between P. betacei and S. betaceum.

plant biology↗

Helper NLRs Nrc2 and Nrc3 act co-dependently with Prf/Pto and activate MAPK signaling to induce immunity in tomato

Plant intracellular immune receptors, primarily nucleotide-binding, leucine-rich repeat proteins (NLRs), can detect virulence proteins (effectors) from pathogens and activate NLR-triggered immunity (NTI). Recently, sensor NLRs have been reported to function with helper NLRs to activate immune responses. We investigated the role of two helper NLRs, Nrc2 (NLR required for cell death 2) and Nrc3, on immunity in tomato to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) mediated by the sensor NLR Prf and the Pto kinase. Loss-of-function mutations in both Nrc2 and Nrc3 completely compromised Prf/Pto-mediated NTI to Pst containing the cognate effectors AvrPto and AvrPtoB. An nrc3 mutant showed intermediate susceptibility between wild-type tomato plants and a Prf mutant, while an nrc2 mutant developed only mild disease symptoms. These observations indicate that Nrc2 and Nrc3 act additively to contribute to Prf/Pto-mediated immunity. We also examined at what point Nrc2 and Nrc3 act in the Prf/Pto-mediated immune response. In the nrc2/3 mutant, programmed cell death (PCD) normally induced by constitutively-active variants of AvrPtoB, Pto or Prf was abolished, but that induced by M3K or Mkk2 was not. PCD induced by a constitutively active variant of Nrc3 was also abolished in a Nicotiana benthamiana line with reduced expression of Prf. MAPK activation triggered by expression of AvrPto in the wild-type Pto-expressing tomato plants was completely abolished in the nrc2/3 mutant. These results indicate that Nrc2 and Nrc3 act in concert with Prf/Pto and upstream of MAPK signaling. Nrc2 and Nrc3 were not required for the HR triggered by Ptr1, another sensor NLR mediating Pst resistance, although these helper NLRs do appear to be involved in resistance to certain Pst race 1 strains.

plant biology↗

Evaluation of small non-coding RNAs as a possible epigenetic mechanism mediating the transition from biotrophy to necrotrophy in the life cycle of Phytophthora infestans

Phytophthora infestans, causal agent of late blight disease of potatoes, causes billion-dollar losses worldwide each year. This plant pathogen is a hemibiotroph, first feeding on the host and later killing it. Even though the transcription dynamics of this transition are characterized, the role that small non-coding RNAs (sRNAs) might have is still unknown. Furthermore, a bioinformatic pipeline to search and analyze sRNAs in P. infestans, is needed. Using our proposed pipeline, 146 sRNAs were found to be significantly differentially expressed between the evaluated stages of the pathogens life cycle. One hundred of these sRNAs were successfully annotated and classified into nine functional categories. The expression of the genes associated to ten of these sRNAs was validated via qRT-PCR. Among these, the expression levels of genes encoding for effectors were inversely correlated to that of the sRNAs aligning to them, which is expected if sRNAs are indeed regulating their expression. This correlation was not clear for sRNAs in other functional categories and should not be confused with strict causality. This study works as a starting point for considering sRNAs as role players in the transition from biotrophy to necrotrophy in P. infestans when infecting Solanum tuberosum.

microbiology↗