Search bioRxiv⌕ Search

Biology subjects

Gohel, C.

Publications and source records attributed to Gohel, C..

2 recordsLinked to original sources

cellSight: Characterizing dynamics of cells using single-cell RNA-sequencing

Single-cell analysis has transformed our understanding of cellular diversity, offering insights into complex biological systems. Yet, manual data processing in single-cell studies poses challenges, including inefficiency, human error, and limited scalability. To address these issues, we propose the automated workflow cellSight, which integrates high-throughput sequencing in a user-friendly platform. By automating tasks like cell type clustering, feature extraction, and data normalization, cellSight reduces researcher workload, promoting focus on data interpretation and hypothesis generation. Its standardized analysis pipelines and quality control metrics enhance reproducibility, enabling collaboration across studies. Moreover, cellSights adaptability supports integration with emerging technologies, keeping pace with advancements in single-cell genomics. cellSight accelerates discoveries in single-cell biology, driving impactful insights and clinical translation. It is available with documentation and tutorials at https://github.com/omicsEye/cellSight.

bioinformatics↗

Deep skin fibroblast-mediated macrophage recruitment supports acute wound healing

Epithelial and immune cells have long been appreciated for their contribution to the early immune response after injury; however, much less is known about the role of mesenchymal cells. Using single nuclei RNA-sequencing, we defined changes in gene expression associated with inflammation at 1-day post-wounding (dpw) in mouse skin. Compared to keratinocytes and myeloid cells, we detected enriched expression of pro-inflammatory genes in fibroblasts associated with deeper layers of the skin. In particular, SCA1+ fibroblasts were enriched for numerous chemokines, including CCL2, CCL7, and IL33 compared to SCA1-fibroblasts. Genetic deletion of Ccl2 in fibroblasts resulted in fewer wound bed macrophages and monocytes during injury-induced inflammation with reduced revascularization and re-epithelialization during the proliferation phase of healing. These findings highlight the important contribution of deep skin fibroblast-derived factors to injury-induced inflammation and the impact of immune cell dysregulation on subsequent tissue repair.

cell biology↗