Gauchan, D. P. | Search bioRxiv

bioRxiv2026

Uncovering bioactive metabolites from the Taxus wallichiana endophyte Annulohypoxylon purpureonitens using reverse metabolomics

Endophytic fungi associated with the Himalayan yew (Taxus wallichiana) represent an underexplored source of bioactive secondary metabolites. This study investigated the extracellular metabolites of Annulohypoxylon purpureonitens isolated from Nepalese T. wallichiana using bioactivity screening combined with LCMS/MS-based metabolomics. The fungal extract exhibited broad-spectrum antibacterial activity, showing the strongest inhibition against Staphylococcus aureusand Enterococcus faecalis (MIC = 500 ug/mL). It also displayed notable antioxidant capacity(DPPH, ABTS, TPC &TFC) and cytotoxicity against HeLa and MCF-7 cancer cell lines. Metabolite profiling via GNPS molecular networking, manual MS/MS validation, and MASST reverse metabolomics putatively identified diverse compounds, including hydroquinidine, chlorogenic acid, muramic acid, and cordycepin conjugates widely distributed across public microbial datasets. Overall, A. purpureonitens is a promising source of multifunctional metabolites, laying a foundation for future compound isolation and functional characterization.

pharmacology and toxicology↗

bioRxiv2020

Annulohypoxylon sp. strain MUS1, an Endophyte isolated from Taxus wallichiana Zucc. produces Taxol and Other Bioactive Metabolites

Endophytes are microbial colonizers that reside in plants by symbiotic association produces several biological classes of natural products. The current study focuses on the isolation and characterization of bioactive compounds produced by endophytic fungi isolated from the Himalayan yew (Taxus wallichiana) collected from the Mustang district of Nepal. The plant materials were collected from the Lower-Mustang region in the month of October 2016 and the endophytic fungi were isolated on artificial media from inner tissues of bark and needles. Antimicrobial and antioxidant activity, along with total phenolic- and flavonoid-content assays, were used in the evaluation of bioactivity of the fermented crude extracts along with the in vitro ability of the endophytes to produce the anticancer compound Taxol was analyzed. A total of 16 fungal morphotypes were obtained from asymptomatic inner tissues of the bark and needles of T. wallichiana. Among the 16 isolates, the ethyl acetate (EA) fraction of isolate MUS1, showed antibacterial and antifungal activity against all test-pathogens used, with significant inhibition against Pseudomonas aeruginosa ATCC 27853 (MIC: 250 {micro}g/ml) and the pathogenic yeast, Candida albicans (MIC: 125 {micro}g/ml). Antioxidant activity was also evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH). At a concentration of 100 {micro}g/ml, the % radical scavenging activity was 83.15{+/-}0.40, 81.62{+/-}0.11, and 62.36{+/-}0.29, for ascorbic acid, butylated hydroxytoluene (BHT) and the EA fraction of MUS1, respectively. The DPPH-IC50 value for the EA fraction was 81.52 {micro}g/ml, compared to BHT (62.87 {micro}g/ml) and ascorbic acid (56.15 {micro}g/ml). The total phenolic and flavonoid content in the EA fraction were 16.90{+/-}0.075 {micro}g gallic acid equivalent (GAE) and 11.59{+/-}0.148 {micro}g rutin equivalent (RE), per mg of dry crude extract, respectively. Isolate MUS1, identified as an Annulohypoxylon sp. by ITS sequencing, also produced Taxol (282.05 {micro}g/L) as shown by TLC and HPLC analysis. Having the ability to produce antimicrobial and antioxidant compounds, as well as the anticancer compound Taxol, makes Annulohypoxylon sp. strain MUS1, a promising candidate for further study given that naturally occurring bioactive compounds are of great interest to the pharmacological, food and cosmetic industries.

microbiology↗