Biology subjects

Gasparoli, F.

Publications and source records attributed to Gasparoli, F..

3 recordsLinked to original sources

bioRxiv2020

Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution

Imaging across length scales and in depth has been an important pursuit of widefield optical imaging. This promises to reveal fine cellular detail within a widefield snapshot of a tissue sample. Current advances often sacrifice resolution through selective sub-sampling to provide a wide field of view in a reasonable time scale. We demonstrate a new avenue for recovering high-resolution images from sub-sampled data in light-sheet microscopy using deep-learning super-resolution. We combine this with the use of a widefield Airy beam to achieve high-resolution imaging over extended fields of view and depths. We characterise our method on fluorescent beads as test targets. We then demonstrate improvements in imaging amyloid plaques in a cleared brain from a mouse model of Alzheimers disease, and in excised healthy and cancerous colon and breast tissues. This development can be widely applied in all forms of light sheet microscopy to provide a two-fold increase in the dynamic range of the imaged length scale. It has the potential to provide further insight into neuroscience, developmental biology and histopathology.

biophysics↗

bioRxiv2019

Multi-photon attenuation-compensated light-sheet fluorescence microscopy

Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an increase in depth penetration for imaging. Here we show this promising approach may be implemented in multi-photon (two-photon) light-sheet fluorescence microscopy and, furthermore, be achieved in a facile manner utilizing a graded neutral density filter, circumventing the need for complex beam shaping apparatus. A "gold standard" system utilizing a spatial light modulator for beam shaping is used to benchmark our implementation. The approach will open up enhanced depth penetration in light-sheet imaging to a wide range of end users.

cell biology↗

bioRxiv2018

The dyslexia susceptibility KIAA0319 gene shows a highly specific expression pattern during zebrafish development supporting a role beyond neuronal migration.

Dyslexia is a common neurodevelopmental disorder that affects reading abilities and is predicted to be caused by a significant genetic component. Very few genetic susceptibility factors have been identified so far and amongst those, KIAA0319 is a key candidate. KIAA0319 is highly expressed during brain development but its function remains poorly understood. Initial RNA-interference studies in rats suggested a role in neuronal migration whereas subsequent work with double knock-out mouse models for both Kiaa0319 and its paralogue Kiaa0319-like reported effects in the auditory system but not in neuronal migration. To further understand the role of KIAA0319 during neurodevelopment, we carried out an expression study of the zebrafish orthologue at different embryonic stages. We report particularly high gene expression during the first few hours of development. At later stages, expression becomes localised in well-defined structures such as the eyes, the telencephalon and the notochord, supporting a role for kiaa0319 that is not restricted to brain development. Surprisingly, kiaa0319-like, which generally shows a similar expression pattern, was not expressed in the notochord suggesting a role specific to kiaa0319 in this structure. This study contributes to our understanding of KIAA0319 function during embryonic development which might involve additional roles in the visual system and in the notochord. Such a specific spatiotemporal expression pattern is likely to be under the controlled of tightly regulated sequences. Therefore, these data provide a framework to interpret the effects of the dyslexia-associated genetic variants that reside in KIAA0319 non-coding regulatory regions.

neuroscience↗