Garrido, S. | Search bioRxiv

bioRxiv2025

Genomic Evidence for Revising Management Units of European Anchovy: Integrating Evolutionary Lineages into Fisheries Assessment

Effective fisheries management requires stock boundaries that accurately reflect underlying biological populations. In European anchovy (Engraulis encrasicolus), defining management units has been complicated by a complex evolutionary history and the coexistence of distinct ecotypes. Despite recent advances using high-resolution genomic markers, key uncertainties persist regarding population structure and connectivity, particularly between the Bay of Biscay and Atlantic Iberian Waters stocks and their links to neighboring northern and southern regions. Here, we analyze thousands of genetic markers from individuals spanning both assessed stocks and adjacent areas, including representatives of the two recognized ecotypes (marine and coastal). Our comprehensive population genomic analyses identify three major lineages, northern marine, southern marine, and coastal, shaped by historical processes and ecological differentiation. Notably, genetic divergence between ecotypes exceeds that observed among geographically distant populations within the same ecotype, highlighting the need to incorporate ecological as well as spatial drivers when delineating stocks. Our findings demonstrate that current management units do not capture the underlying biological structure of European anchovy, which could lead to local overexploitation due to inadequate Total Allowable Catch (TAC) settings. This study emphasizes the need to incorporate genetic data when defining biologically relevant management units, with the goal of improving stock assessments, safeguarding adaptive potential, and ensuring the long-term sustainability of the species.

evolutionary biology↗

bioRxiv2025

Inversions Dominate Evolution in the Highly Admixed European Sardine (Sardina pilchardus)

Inversions can play key roles in the genetic architecture of adaptation, but the scale of their effects across different species remains poorly understood. Here, we use whole- genome sequencing to investigate the influence of inversions on the population genomics of the r-selected European sardine (Sardina pilchardus). Allele frequency differences from millions of SNPs across 34 populations spanning the species range were analyzed. Genomic scans identified several extreme outlier regions overlapping chromosome-scale inversions, collectively representing over half the genome. Our findings suggest these inversions are associated with locally adapted life history strategies. First, SNPs within outlier regions containing inversions exhibited striking allele frequency differences between Atlantic and Mediterranean sardines, which differ in key adaptive life history traits. In the Atlantic, inversion allele frequencies varied latitudinally, while in the Mediterranean, they shifted longitudinally, aligning with temperature and oceanographic features that influence sardine life history strategies. Moreover, adjacent populations in contrasting environments displayed pronounced allele frequency differences in inversions. These spatial patterns of allele frequencies sharply contrasted with those based on neutral loci, indicating they are driven by selection. After rigorously filtering SNPs affected by selection and inversions, sardine populations showed high admixture across their range but significant population structure and isolation by distance, especially in the Mediterranean. This study demonstrates that inversions can shape genome-wide patterns of genetic diversity and population structure in highly admixed r-selected marine species. These findings also offer crucial insights for stock delimitation and management of this commercially valuable species in the face of climate change.

genomics↗