Search bioRxiv⌕ Search

Biology subjects

Garofalo, S.

Publications and source records attributed to Garofalo, S..

2 recordsLinked to original sources

Environmental volatility amplifies Pavlovian control in decision-making

Volatile environments require continuous updating of action values, making instrumental knowledge less informative. Under such conditions, Pavlovian control may offer a more stable alternative, since the value attributed to reward-predictive cues is not altered by continuously updated instrumental predictions. Using a modified Pavlovian-to-Instrumental Transfer paradigm in two preregistered experiments, we test the hypothesis that increased volatility promotes the recruitment of Pavlovian control, whereby reward-predictive cues bias choice to the predicted outcome, simplifying (and possibly overcoming) the high computational cost of the instrumental prediction. Crucially, using entropy-based metrics we demonstrate that Pavlovian control is not unitary, but can instead respectively increase or reduce entropy depending on whether the choice outcome is present or not. Together, these findings show that volatility redirects control in Pavlovian-instrumental arbitration toward the cues, rather than merely adding noise to behaviour.

animal behavior and cognition↗

Microglia control glutamatergic synapses in the adult mouse hippocampus

Microglial cells are active players in regulating synaptic development and plasticity in the brain. However, how these cells influence the normal functioning of synapses is largely unknown. In this study, we characterized the effects of pharmacological depletion of microglia, achieved by administration of PLX5622, on hippocampal CA3-CA1 synapses of adult wild type mice. Following microglial depletion, we observed a reduction of spontaneous and evoked glutamatergic activity associated with a decrease of dendritic spine density. We also observed the appearance of immature synaptic features accompanied by higher levels of plasticity. In addition, microglia depleted mice showed a deficit in the acquisition of the Novel Object Recognition task. Remarkably, microglial repopulation after PLX5622 withdrawal was associated with the recovery of hippocampal synapses and learning functions. Altogether, these data demonstrate that microglia contribute to normal synaptic functioning in the adult brain and that their removal induces reversible changes in synaptic organization and activity of glutamatergic synapses.

neuroscience↗