Biology subjects

Garcia-delaTorre, P.

Publications and source records attributed to Garcia-delaTorre, P..

6 recordsLinked to original sources

bioRxiv2023

Physical performance and telomere length in older adults

BackgroundThe fast-paced aging demographic prompts studying risk factors and markers that could predict healthy aging. Telomere length shows promise for assessing a broad spectrum of aging-related phenotypes. Aim of the studyTo assess the association between physical performance and telomere length in Mexican older adults. MethodsIn this observational cross-sectional study, we recruited 323 older adults affiliated with the Instituto Mexicano del Seguro Social (IMSS) and members of the "Cohort of Obesity, Sarcopenia, and Frailty of Older Mexican Adults (COSFOMA). To assess physical performance, we used the Short Physical Performance Battery (SPPB) and stratified individuals into two groups according to their SPPB score into low [≤]7 (L-SPPB) and high >7 (H-SPPB). Absolute telomere length (aTL) was determined by using qPCR. Next, we classified individuals according to their aTL into short [≤]4.22 kb (S-TL) and long >4.22 kb (L-TL). For both SPPB and aTL categories, we calculated the mean and adjusted mean by sex, age, marital status, education, remunerated work, smoking, drinking, cognitive decline, depression, and polypharmacy with 95% CI, including the lower and upper CI (LCImeanUCI). We estimated the effect size between physical performance and telomere length with Cohens d for unequal group sizes. Also, we calculated the odds ratio with 95% confidence intervals, including the lower and upper CI (LCIORUCI) for physical performance according to telomere length categories. ResultsParticipants in the low physical performance category had significantly shorter telomeres (4.14.44.7 mean and 3.54.04.5 kb adjusted mean, p<0.001), in opposition to the high physical performance category (score >7) with longer telomeres (5.55.75.9 mean and 4.75.35.8 kb adjusted mean, p<0.001), with a medium-to-high telomere length effect size (d= 0.762). Finally, the odds of being classified in the low physical activity category increased 2.13.66.1 times per kb of telomere (adjOR 1.73.36.3, p<0.001) compared to the high physical activity group (p<0.001). ConclusionDecreased physical functioning is associated with lower telomere length. Absolute telomere length (aTL) as a possible biomarker for differential diagnosis of healthy and unhealthy aging should be explored further.

genetics↗

bioRxiv2020

Citrulline supplementation improves spatial memory in a murine model for Alzheimer's disease

BackgroundAlzheimers disease (AD) correlates with the dysfunction of metabolic pathways that translates into neurological symptoms. An arginine deficiency, a precursor of nitric oxide (NO), has been reported for AD patients. ObjectiveWe aimed to evaluate the effect of citrulline oral supplementation on cognitive decline in an AD murine model. MethosThree-month citrulline or water supplementation was blindly given to male and female wild type and 3xTg-AD mice trained and tested in the Morris Water Maze (MWM). Cerebrospinal fluid and brain tissue were collected. Ultra Performance Liquid Chromatography was used for arginine determinations while the Griess method was used for NO. ResultsEight-month-old male 3xTg-AD mice supplemented with citrulline performed significantly better in the MWM task; arginine levels increased in cerebrospinal fluid although no changes were seen in brain tissue and only a tendency of increase of NO was observed. ConclusionsCitrulline oral administration is a viable treatment for memory improvement in the early stages of AD pointing to NO as a viable, efficient target for memory dysfunction in AD.

neuroscience↗

bioRxiv2019

Telomere length and oxidative stress in the progression of Alzheimer’s disease

Alzheimers Disease (AD) is the most common cause of dementia and aging is its major risk factor. Changes in telomere length have been associated with aging and some degenerative diseases. Our aim was to explore some of the molecular changes caused by the progression of AD in a transgenic murine model (3xTg-AD; B6; 129-Psen1 Tg (APPSwe, tauP301L) 1Lfa). Telomere length was assessed by qPCR in both brain tissue and peripheral blood cells and compared between three age groups: 5, 9, and 13 months. In addition, a possible effect of oxidative stress on telomere length and AD progression was explored. Shorter telomeres were found in blood cells of older transgenic mice compared to younger and wild type mice but no changes in telomere length in the hippocampus. An increase in oxidative stress with age was found for all strains but no correlation was found between oxidative stress and shorter telomere length for transgenic mice. Telomere length and oxidative stress are affected by AD progression in the 3xTg-AD murine model. Changes in blood cells are more noticeable than changes in brain tissue, suggesting that systemic changes can be detected early in the disease in this murine model.

neuroscience↗

bioRxiv2018

Association of oxidative stress, telomere length, and frailty in an elder population

BackgroundA global aging population requires focusing on the risk factors for unhealthy aging, preventive medicine, and chronic disease management. The identification of adverse health outcomes in older adults has been addressed by the characterization of frailty as a biological syndrome. On the other hand, oxidative stress and telomere length have been suggested as biomarkers of aging.\n\nObjectiveTo study the association of oxidative stress, telomere length, and frailty in an old age population.\n\nMethodsThis was a cross-sectional study based on 2015 data from 202 members from a cohort of older adults (n=202; gender F/M ratio: 133/69; mean age: 69.89 {+/-} 7.39 years). Reactive oxygen species (ROS) were measured by dichlorofluorescin diacetate, and lipid peroxidation by malondialdehyde. Telomere length was determined using qPCR with SYBR Green Master Mix.\n\nResultsStatistical analysis showed an association between telomere length and frailty but no association between oxidative stress on telomere length or frailty.\n\nConclusionsTelomere length could eventually be used as a marker to discriminate between healthy and unhealthy aging as expressed by frailty phenotype. However, oxidative stress seems as just a biological process of aging.

molecular biology↗

bioRxiv2018

Oxidative stress in an aged population with diabetes mellitus and/or hypertension

BackgroundMexico City has the highest aging rate in the country, as well as a high prevalence of diabetes mellitus (DM) and arterial hypertension (HT). All three on their own, are known to increase oxidative stress (OE).\n\nMethodsFinal groups included 18 patients without DM or HT (control group), 12 with DM, 23 with HT, and 18 with DM and HT. The EO was measured by the quantification of reactive oxygen species (ROS), and by determination of lipid peroxidation.\n\nResultsHAS patients showed increased ROS levels as did men with HAS compared with the respective DM and HT groups. Also, women of the control group showed higher levels of ROS compared with men. HT in an aged population turned out to be the most influential factor for oxidative stress increase while DM had no effect whatsoever.

epidemiology↗

bioRxiv2017

BDNF and Tau as biomarkers of severity in Multiple Sclerosis

AimsDetermine if serum levels of tau and brain-derived neurotrophic factor (BDNF) can be used as severity biomarkers in multiple sclerosis (MS).\n\nPatients and methodsSubjects with MS, older than 18 and younger than 55 years were included; 74 patients with a diagnosis of relapsing-remitting MS (RRMS), 11 with secondary-progressive MS (SPMS), and 88 controls were included. Total tau and BDNF were measured by western blot.\n\nResultsIncreased tau and decreased BDNF in MS patients compared to controls was found. Total-tau has a peak in RRMS, the second decile of the Multiple Sclerosis Severity Score, and in the lowest Expanded Disability Status Scale and is no different than controls for SPMS patients and the most severe cases of MS.\n\nConclusionBDNF is a good biomarker for diagnosis of MS but not for severity or progression. Tau appears to have a more active role in the progression of MS.

neuroscience↗