Biology subjects

Frawley, E.

Publications and source records attributed to Frawley, E..

3 recordsLinked to original sources

bioRxiv2022

Temporal and environmental factors interact with rootstock genotype to shape leaf elemental composition in grafted grapevines

Plants take up elements through their roots and transport them to their shoot systems for use in numerous biochemical, physiological, and structural functions. Elemental composition of above-ground plant tissues, such as leaves, reflects both above- and below-ground activities of the plant genotype, as well the local environment. Perennial, grafted plants, where the root system of one individual is fused to the shoot system of a genetically distinct individual, offer a powerful experimental system in which to study the role of the root system in the elemental composition of the shoot system. We measured elemental composition of over 7000 leaves in the grapevine cultivar Chambourcin growing ungrafted and grafted to three rootstock genotypes. Leaves were collected over multiple years and phenological stages (across the season) and along a developmental time series. Temporal components of this study had the largest effect on leaf elemental composition; and rootstock genotype interacted with year, phenological stage, and leaf age to differentially modulate leaf elemental composition. Further, the local, above-ground environment affected leaf elemental composition, an effect influenced by rootstock genotype. This work highlights the dynamic nature by which root systems interact with shoot systems to respond to temporal and environmental variation.

plant biology↗

bioRxiv2020

Root system influence on high dimensional leaf phenotypes over the grapevine growing season

O_LIIn many perennial crops, grafting the root system of one individual to the shoot system of another individual has become an integral part of propagation performed at industrial scales to enhance pest, disease, and stress tolerance and to regulate yield and vigor. Grafted plants offer important experimental systems for understanding the extent and seasonality of root system effects on shoot system biology. C_LIO_LIUsing an experimental vineyard where a common scion Chambourcin is growing ungrafted and grafted to three different rootstocks, we explore associations between root system genotype and leaf phenotypes in grafted grapevines across a growing season. We quantified five high-dimensional leaf phenotyping modalities: ionomics, metabolomics, transcriptomics, morphometrics, and physiology and show that rootstock influence is subtle but ubiquitous across modalities. C_LIO_LIWe find strong signatures of rootstock influence on the leaf ionome, with unique signatures detected at each phenological stage. Moreover, all phenotypes and patterns of phenotypic covariation were highly dynamic across the season. C_LIO_LIThese findings expand upon previously identified patterns to suggest that the influence of root system on shoot system phenotypes is complex and broad understanding necessitates volumes of high-dimensional, multi-scale data previously unmet. C_LI

plant biology↗

bioRxiv2019

Building a botanical foundation for perennial agriculture: Global inventory of wild, perennial herbaceous Fabaceae species

O_LIConcerns about soil health and stability are focusing attention on crops that deliver both agricultural products and ecological services. Deep rooted, perennial plants that build soil organic matter, support diverse below-ground microbial communities, and produce edible seeds are key components underpinning ecological intensification; however few perennial, herbaceous crops have been domesticated for food.\nC_LIO_LITo facilitate development of edible, perennial, herbaceous crops, including perennial grains, we constructed an online resource of wild, perennial, herbaceous species - the Perennial Agriculture Project Global Inventory (PAPGI; http://www.tropicos.org/Project/PAPGI). The first component of this project focuses on wild, perennial, herbaceous Fabaceae species. We extracted taxonomic names and descriptors from the International Legume Database and Information Service. Names were added to PAPGI, a special project within the botanical database TROPICOS, where they link to specimen records and ethnobotanical and toxicological data. PAPGI includes 6,644 perennial, herbaceous Fabaceae species. We built a searchable database of more than 60 agriculturally important traits. Here we highlight food and forage uses for 314 legume species, and toxicological data for 278 species.\nC_LIO_LIThe novel contribution of PAPGI is its focus on wild, perennial herbaceous species that generally have not entered the domestication process but that hold promise for development as perennial food crops. By extracting botanical information relevant for agriculture we provide a dynamic resource for breeders and plant scientists working to advance ecological intensification of agriculture, and for conservation managers working to preserve wild species of potential agricultural importance.\nC_LI\n\nSocietal Impact StatementAgroecosystems are constantly evolving to meet the needs of a growing population in a sustainable manner. Perennial, herbaceous crops deliver both agricultural products and ecological services. Until recently, edible, perennial, herbaceous crops, including perennial grains, were absent from agriculture. Perennial, herbaceous crops can be developed through wide hybridization between annual crops and perennial relatives or by de novo domestication of wild species. The diversity of wild, perennial, herbaceous legume species documented by the PAPGI increases resources available to breeders of perennial, herbaceous legumes, and raises awareness about previously untapped wild plant diversity in future crop development.

evolutionary biology↗