Francini, A. | Search bioRxiv

bioRxiv2025

High-Quality PacBio Genome Assembly of Populus alba L. Villafranca

This study presents the high-quality genome assemblies for Populus alba L. "Villafranca" using PacBio HiFi sequencing. The assembly span 498.95 Mb, an N50 of 18.18 Mb and largest contig of 52.03 Mb. BUSCO analysis revealed genome completeness (embryophyta_odb10) with 98.8% of the 1,614 BUSCO groups searched. The Transposable element and repetitive content accounted for [~]31.37%. The comparison of P. alba and P. trichocarpa genomes identified 9,741 structural variants (SVs) This comprehensive analysis provides valuable resources for studying poplar genome evolution, domestication, and genetic improvement, underscoring the utility of long-read sequencing for resolving complex genomic features.

genomics↗

bioRxiv2024

PacBio genome assembly of Olea europaea L. subsp. europaea cultivars 'Frantoio' and 'Leccino' reveal main structural differences in key genes related to salt stress

We present two high-quality genome assemblies for Olea europaea L. cultivars Frantoio and Leccino leveraging PacBio HiFi sequencing to achieve approximately 30x genome coverage for each cultivar. The assemblies span 1.18 Gbp and 1.43 Gbp with contig N50 values of 1.78 Mbp and 45.88 Mbp for Frantoio and Leccino respectively. BUSCO analysis revealed a great genome completeness ([~]97.9%), surpassing many of earlier Olea europaea assemblies and is in par with the most recent one. Repetitive content accounted for [~]67.5% in Frantoio and [~]70.8% in Leccino with long terminal repeats (LTRs) dominating. Notably, a tandem repeat family, Satellite 1, represented [~]16.9% and [~]8.6% of the Leccino and Frantoio genomes, respectively. The structural variant (SV) analysis was done with a particular focus on those associated with nine key gene families involved in salinity tolerance and identified cultivar-specific genomic differences, emphasizing the diversity within domesticated olives. This comprehensive analysis provides valuable resources for studying olive genome evolution, domestication, and genetic improvement, underscoring the utility of long-read sequencing for resolving complex genomic features.

genomics↗