miR-196a-5p and miR-342-3p mediate skeletal muscle and thermogenic adipose tissue crosstalk through extracellular vesicles
Small extracellular vesicles (small EVs) are nanovesicles found in tissues and body fluids that contain regulatory molecules including microRNAs, termed exomiRs. Research in murine models has demonstrated that exercise can trigger the release of small EVs into the circulation. The aim of this study was to study exomiR release in humans pre and post exercise and to characterise the function of these microRNAs especially in relation to thermogenic fat. We found that exercise increased the release of exomiR-196a-5p in endurance athletes, a microRNA that induces UCP1 expression and browning of white adipocytes. We observed that myotubes specifically release miR-196a-5p within small EVs after in vitro exercise-mimicking conditions such as electrical pulse stimulation and cAMP treatment. Likewise, the expression at basal levels of the exercise-induced exomiR-342-3p negatively correlated with BMI and age. EV proteomics revealed a positive correlation between FABP4+ and miR-342-3p, suggesting an adipocyte cell origin. Overexpression of miR-342-3p increased Myogenin levels during skeletal muscle cell differentiation, indicating a positive role in muscle differentiation. Our results suggest that oxidative extreme metabolic capacities in endurance athletes contribute to the enhanced release of circulatory exomiRs after exercise mediating bi-directional crosstalk between skeletal muscle and thermogenic adipose tissue. Graphical abstractO_LISerum-EVs from endurance athletes increase UCP1 expression in white adipocytes. C_LIO_LImiR-196a and miR-342-3p are increased in serum-EVs from endurance athletes. C_LIO_LIMuscle cells release EVs enriched in miR-196a after electrical pulse stimulation. C_LIO_LImiR-196a and miR-342-3p have browning and myogenic potential, respectively. C_LI O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=143 SRC="FIGDIR/small/656129v1_ufig1.gif" ALT="Figure 1"> View larger version (31K): org.highwire.dtl.DTLVardef@1bd9b25org.highwire.dtl.DTLVardef@114a549org.highwire.dtl.DTLVardef@6f863borg.highwire.dtl.DTLVardef@1d5c528_HPS_FORMAT_FIGEXP M_FIG C_FIG