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Biology subjects

Ferreira, L. A.

Publications and source records attributed to Ferreira, L. A..

2 recordsLinked to original sources

Acetaminophen attenuates pathological pain through a mechanism that requires CB1 cannabinoid receptors and the enzyme diacylglycerol lipase in mice

Acetaminophen (APAP) produces analgesia through mechanisms that remain poorly understood. Here, we tested the hypothesis that APAP-induced suppression of pathological pain is associated with cannabinoid receptors and activity of enzymes regulating endogenous lipids (diacylglycerol lipase, DAGL; monoacylglycerol lipase, MAGL) including endocannabinoids. APAP suppressed mechanical hypersensitivity in mouse models of post-surgical and inflammatory pain and the DAGL inhibitors (RHC-80267, DO34) and MAGL inhibitor JZL184 blocked APAPs analgesic effects. Global (rimonabant, AM251) but not peripherally restricted (AM6545) cannabinoid receptor antagonists prevented APAP-induced analgesia. APAP increased corticosterone levels >2-fold and reduced prostaglandins >5-fold across the brain and in the paw skin. In addition, APAP reduced up to 39% of signaling lipids detected in the targeted screen in CFA-treated subjects in a tissue-dependent manner. These observations suggest that APAP plays a wider role in endogenous lipid signaling than previously hypothesized and provides novel insight into mechanisms of action.

neuroscience↗

The future is fully defined: recombinant fragment E8 of laminin-511 is a viable xenofree alternative to Matrigel for hiPSC culture and differentiation into neurovascular cell types

Matrigel remains the gold standard substrate for culture of induced pluripotent stem cells (iPSCs). However, its highly variable composition, animal origin and unpredictable effects on biological activity have been discussed for more than 3 decades. In this study, we explore the use of fragment E8 of recombinant laminin 511, commercially available in form of iMatrix-511, as an alternative to Matrigel for iPSC maintenance and differentiation. Female iMR90-4 human iPSCs were cultured on either iMatrix or Matrigel and assessed for cell growth and viability, pluripotency, genetic stability, and ability to differentiate into isogenic brain microvascular endothelial cells (iBMECs) and brain pericytes. It was observed that iMatrix facilitated iPSC growth and viability comparable to Matrigel while maintaining a higher number of more consistently sized colonies. Additionally, like Matrigel, iMatrix maintained the expression of pluripotency markers SSEA-4 and OCT-3/4 over 15 passages without inducing DNA damage. iMatrix also supported the differentiation of these iPSCs into isogenic iBMECs and pericytes, which were successfully co-culture for generation of a simplified blood-brain barrier model. Overall, we showed that iMatrix, which is a cost effective, fully defined, and xenofree alternative can be used as a substitute for Matrigel for maintenance and differentiation of iPSCs.

bioengineering↗