Biology subjects

Ferdous, R.

Publications and source records attributed to Ferdous, R..

3 recordsLinked to original sources

bioRxiv2025

Optimizing brinjal (Solanum melongena L.) health and yield through bio-organic amendments against Fusarium wilt

A field experiment was conducted to evaluate the effect of different bio-organic treatments, including control (T0), spent mushroom substrate-SMS (T1), vermicompost (T2), poultry manure (T3), biochar (T4), SMS with biochar (T5), SMS with poultry manure (T6), and SMS with vermicompost (T7) on the management of Fusarium wilt of brinjal (Solanum melongena L.). Pathogen identification confirmed Fusarium oxysporum f. sp. melongenae as the causal agent of wilt through morphological and pathogenicity tests. Growth attributes, yield, and disease incidence were recorded at various growth stages. The lowest disease incidence (5.55%) was observed in T5, followed by T6, while the highest disease incidence (44.4%) was found in the untreated control (T0). The treatment of T5 significantly enhanced plant growth parameters, including plant height, number of leaves, and number of branches, alongside a substantial increase in yield (12.71 tons/ha), which was statistically similar to T6. These bio-organic amendments not only suppressed disease incidence effectively but also improved soil health, enhanced microbial diversity, and promoted vegetative growth and yield. The results indicate that the integration of SMS with Biochar or Poultry Manure is a sustainable and eco-friendly strategy for managing Fusarium wilt in brinjal cultivation, potentially replacing conventional chemical methods for enhanced productivity and soil health.

microbiology↗

bioRxiv2024

Natural field diagnosis and molecular confirmation of fungal and bacterial watermelon pathogens in Bangladesh: A case study from Natore and Sylhet district

The study investigated watermelon diseases in Sylhet and Natore Districts of Bangladesh, characterized by contrasting climatic conditions. Sylhet experiences lower temperatures and high rainfall, while Natore has higher temperatures and low rainfall. In these survey regions, 40 watermelon fields were selected, and 10 diseases were observed, including 4 fungal, 3 bacterial, 2 water mold, and 1 viral disease. The observed diseases were Anthracnose, Cercospora leaf spot, Fusarium wilt, Gummy stem blight, Downy mildew, Phytophthora fruit rot, Bacterial fruit blotch, Angular leaf spot, Yellow vine, and Watermelon mosaic disease. Molecular analysis was done in the Plant Pathology Lab at Sher-e-Bangla Agricultural University using the specific primers for fungal (ITS1/ITS4) and bacterial (27F/1492R) DNA regions and identified nine pathogen species, excluding the causal organism of the viral disease. The identified pathogens included Colletrotrichum orbiculare, Cercospora citrullina, Fusarium oxysporum, Stagonosporopsis cucurbitacearum, Pseudoperonospora cubensis, Phytophthora capsici, Acidovorax citrulli, Pseudomonas syringae, and Serratia marcescens. The sequencing of the identified pathogens revealed high homology (98.91-99.71%) with known sequences in the GenBank database. Phylogenetic analysis showed six clusters for fungal and water mold pathogen isolates and three for bacterial isolates where the percentages of replicate trees were 100% in all the cases. Among the identified diseases, the highest disease occurrence showed by Fusarium wilt (47.5%) following Gummy stem blight (41.5%) in the Sylhet region while Angular leaf spot (37.5%) followed Yellow vine (33%) in the Natore area. Fusarium wilt also showed high disease intensity showcasing its devastating impact on yield. The study highlights the influence of environmental conditions on disease prevalence and underscores the need for tailored management strategies. These findings provide a foundation for developing targeted disease management practices for sustainable watermelon cultivation in Bangladesh.

pathology↗

bioRxiv2024

Comparative analyses of adsorbed circulating proteins in the PMMA and PES hemodiafilters in patients on predilution online hemodiafiltration

Acute and chronic inflammation are common in patients with end-stage kidney disease (ESKD). So, the adsorption of pro-inflammatory cytokines by the hollow fiber has been expected to modify the inflammatory dysregulation in ESKD patients. However, it remains to be determined in detail what molecules of fiber materials can preferably adsorb proteins from the circulating circuit. We aimed this study to analyze directly the adsorbed proteins in the polymethyl methacrylate (PMMA) and polyethersulfone (PES) membranes in patients on predilution online hemodiafiltration (OL-HDF). To compare the adsorbed proteins in the PMMA and PES hemodiafilters membrane, we initially performed predilution OL-HDF using the PES (MFX-25Seco) membrane while then switched to the PMMA (PMF-A) membrane under the same condition in three patients. We extracted proteins from the collected hemodiafilters by extraction, then SDS-PAGE of the extracted sample, protein isolation, in-gel tryptic digestion, and nano-LC MS/MS analyses. The concentrations of adsorbed proteins from the PMMA and PES membrane extracts were 35.6{+/-}7.9 {micro}g/{micro}L and 26.1{+/-}9.2 {micro}g/{micro}L. SDS-PAGE analysis revealed distinct variations of adsorbed proteins mainly in the molecular weight between 10 to 25 kDa. By tryptic gel digestion and mass spectrometric analysis, the PMMA membrane exhibited higher adsorptions of {beta}2 microglobulin, dermcidin, retinol-binding protein-4, and lambda-1 light chain than those from the PES membrane. In contrast, amyloid A-1 protein was adsorbed more potently in the PES membrane. Western blot analyses revealed that the PMMA membrane adsorbed interleukin-6 (IL-6) approximately 5 to 118 times compared to the PES membrane. These findings suggest that PMMA-based OL-HDF therapy may be useful in controlling inflammatory status in ESKD patients.

pathology↗