Biology subjects

Fejer, G.

Publications and source records attributed to Fejer, G..

3 recordsLinked to original sources

bioRxiv2021

Lysophosphatidylcholine acyltransferase 2 (LPCAT2) Regulates The Expression of Macrophage Phenotype Markers in RAW264.7 Cells.

1Macrophages are key antigen presenting cells that also secrete cytokines during inflammation. They can be polarised to M1 or M2 phenotypes. Molecules such as CD206 and inducible Nitric Oxide Synthase are considered macrophage phenotype markers because they are highly expressed in either M1 or M2 macrophages. LPCAT2 is a phospholipid modifying enzyme that influences inflammatory responses in macrophages. However, how LPCAT2 influences inflammation is not fully understood. In this study, we have used genetic technology to study the influence of LPCAT2 on macrophage phenotype markers. Our results show for the first time that overexpression of LPCAT2 promotes the expression of M1 macrophage phenotype markers, and attenuates the expression of M2 macrophage markers.

immunology↗

bioRxiv2021

Possible Regulation of Toll-Like Receptor 4 By Lysine Acetylation Through LPCAT2 Activity in RAW264.7 Cells.

1Inflammation is central to several diseases. TLR4 mediates inflammatory signals, however, there are gaps in the understanding of its mechanisms. Recently, TLR4 was found to co-localise with LPCAT2, a lysophospholipid acetyltransferase. This interaction influenced TLR4 subcellular localisation through an unknown mechanism. In this study, we have combined computational analysis, RNA interference technology, and biochemical analysis to investigate the possibility of TLR4 lysine acetylation and the influence of LPCAT2 on the detected lysine acetylation. The results suggest for the first time that TLR4 can undergo lysine acetylation and LPCAT2 can influence TLR4 lysine acetylation. This lays a foundation for further research on the role of lysine acetylation on TLR4 and characterisation of LPCAT2 as a protein acetyltransferase.

biochemistry↗

bioRxiv2019

Oropouche virus cases identified in Ecuador using an optimised rRT-PCR informed by metagenomic sequencing

Oropouche virus (OROV) is responsible for outbreaks of Oropouche fever in parts of South America. We recently identified and isolated OROV from a febrile Ecuadorian patient, however, a previously published rRT-PCR assay did not detect OROV in the patient sample. A primer mismatch to the Ecuadorian OROV lineage was identified from metagenomic sequencing data. We report the optimisation of an rRT-PCR assay for the Ecuadorian OROV lineage, which subsequently identified a further five cases in a cohort of 196 febrile patients. We isolated OROV via cell culture and developed an algorithmically-designed primer set for whole-genome amplification of the virus. Metagenomic sequencing of the patient samples provided OROV genome coverage ranging from 68 - 99%. The additional cases formed a single phylogenetic cluster together with the initial case. OROV should be considered as a differential diagnosis for Ecuadorian patients with febrile illness to avoid mis-diagnosis with other circulating pathogens.

genomics↗