Biology subjects

Farber, B.

Publications and source records attributed to Farber, B..

4 recordsLinked to original sources

bioRxiv2020

Improving vaccine efficacy based on non-covalent photoinactivation of microorganisms - sources vaccine antigens (MSVA)

One of the most promising methods for non-covalent inactivation of vaccine-producing microorganisms is the use of photoinactivation using riboflavin derivatives. The study used a dynamic combinatorial derivative of riboflavin - succinyl-maleinyl riboflavin. Corpuscular vaccines are divided into the following groups: from 2AB to 5AB - bacteria were inactivated by riboflavin derivative and blue light, and groups from 6AB to 9AB were inactivated by formalin (0.1% formalin in 9 log CFU was kept for 2 weeks in an thermostat and then sterility was determined - bacterial growth was not observed). A dynamic derivative of riboflavin at a final concentration of 0.02% can photo inactivate 6 time more bacteria P. Aeruginosa and E. coli than riboflavin. The minimum effective blue light emitter power (450 nm) for the photodynamic inactivation of both P. aeruginosa and E. coli is 1024.2 mW / cm2. In groups of mice pre-vaccinated intraperitoneally with corpuscular photo inactivated vaccines based on suspensions of and E. coli at doses of 0.5-1.0 ml 4 log (CFU) / mL, 100% survival of all animals was observed, whereas in control group with the same type of vaccines but formalin-treated vaccines, it failed to achieve a 100% protective effect.

microbiology↗

bioRxiv2019

Pharmacological efficacy of a synergistic composition based on activators of cAMP accumulation as a wound healing agent

IntroductionWound-healing dipyridamole- and papaverine-based aerosols (D1/D2) as activators of the accumulation of cyclic adenosine monophosphate are promising drugs that can accelerate wound healing in wound processes of various origins.\n\nMaterials and methods128 rats were used in the study, including 38 in a pharmacological experiment on a model of stencil wounds and 90 in an experiment that studied the effect of spray on the number of CD34 cells in the blood of rats with chemically induced immunodeficiency. Immunodeficiency was caused by the fivefold administration of cyclophosphamide and prednisone. The expression level of CD34 was determined using flow cytofluorimeter.\n\nResults and discussionTopical application of D1/D2 aerosol samples on the skin of rats contributed to a statistically significant acceleration of regeneration processes. In terms of the appearance of granulations and epithelialization of wounds, D1/D2 aerosols were superior to dexpanthenol ointment. The maximum effect from the use of D1/D2 was observed on the 60th day, and restoration of the physiological level of pluripotent cells was observed as early as on the 10th day after the start of spray application. By accelerating wound healing, dipyridamole with papaverine probably stimulate the division of stem cells at the periphery without enhancing bone marrow function.

pharmacology and toxicology↗

bioRxiv2017

The anticancer activity and immune modulating properties of combinatorial ensemble complementary (antisense) microRNA (fRNA) in combination with the immunomodulator - glycoproteid lectin from B. subtilis B-7025

BackgroundMany adenocarcinomas have the ability to capture from an extracellular matrix the oligonucleotides and nanoparticles by pinocytosis, when the non-cancerous cells are not capable to capture the oligonucleotides and small liposomes. This provides selective accumulation of proposed protected oligonucleotides (fRNA) in cancer cells and also provides the absence toxicity in the fRNA.\n\nMaterials and methodsFor the immunotherapy we used immunotropic 70 kDa lectin B. subtilis B-7025. In vivo experiments were carried out in C57BL line mice in Lewis lung carcinoma. The cytotoxic activity, lymphocytes, macrophages was determined in vitro using the MTT assay.\n\nResults and discussionAnimal survival rate in groups receiving either the fRNA or Vaccine was 70 and 40%, respectively. Combined use fRNA and Vaccine has the advantage compared with the use these drugs in monotherapy, as the anticancer efficacy of the scheme is much higher, which is manifested in the primary tumor node and metastasis inhibition.

cancer biology↗

bioRxiv2017

Influence Of Non-Metabolic Microbial Growth Promotors (AMP Activators) On The Sensitivity To Antimicrobials In The Actually Multiresistant Microbial Strains

IntroductionThe control over multi-resistant nosocomial strains of microorganisms has been becoming increasingly urgent in recent years. We suggest a new paradigm that eliminates killing or inhibiting the growth of bacteria. Excluding bacteria death supresses the selection of resistant strains of microorganisms. We have developed such non-metabolite growth promoters, which in very low doses stimulate the rapid growth of many bacteria strains. The mechanism of action of the enhancers is caused by the activation of the cAMP high doses accumulation process in the microbial cells. cAMP itself is a substrate for phosphorylation including DNA polymerases.\n\nMaterials and methodsThe susceptible culture collection resistance strains Pseudomonas aeruginosa MDR Kharkov IMI1, Acinetobacter baumanii MDR Kharkov-IMI1, and Clebsiella pneumonia MDR Kharkov-IMI1 were used. The following antimicrobial agents of known potency were evaluated: ciprofloxacin, polymyxin, amikacin. The same broth, but containing 0.001% enhancers (under patenting), has been used for further passaging for MDR strains. Characteristics of bacterial growth were determined in a medium compared at the control group - the broth without enhancers.\n\nResults and discussionEnhancers contribute to a significant increase in the antimicrobial sensitivity to polymyxin, ciprofloxacin and amikacin in multi-resistant strains of bacteria. Changes in the growth characteristics and antimicrobial sensitivity are observed only in the second passage that demonstrates the need for the further studies of the molecular mechanisms of the cAMP effect on the division and growth of microbial cells.

microbiology↗