Biology subjects

Elhasan, L. M.

Publications and source records attributed to Elhasan, L. M..

3 recordsLinked to original sources

bioRxiv2022

The Effect of Ethanolic Extract of Acacia Seyal Bark on Induced Diabetic rats

BackgroundDiabetes mellitus Type 2 is a chronic metabolic disorder characterized by insulin insensitivity that leads to a decrease in glucose transport into the other cells. Many drugs are being developed, while no cure is available regarding to this disease although, there are limitations due to high cost and certain side effects. The Traditional Medicines are preferred due to lesser side effects and low cost. Acacia species have wide traditional medicinal using as anti-diabetic, antimicrobial, and anti-inflammatory activities. ObjectiveThe study aimed to examine the effect of ethanolic extract of Acacia Seyal bark in induced diabetic rats. Materials and methodsAcacia Seyal bark was extracted in ethanol 80%. The Ethanolic extract was analyzed for phytochemicals screening tests using standard methods. To investigate the effect of the extract thirty induced diabetic albino rats introduced by injection of glucose 2g\kg were divided to 5 groups equally; Group 1 was treated with 10mg\kg of glibenclamide, group 2 left as control treated with distilled water 10mg\kg, Group 3 was treated with 200mg of plant extract, Group 4 was treated with 400mg of plant extract, and Group 5 was treated with 800mg of plant extract. The Glucose Tolerant Test were done after 1 hour, 2 hour and 4 hour to determine blood glucose level of rats. Estimation of in vitro glucose uptake by rat diaphragm experiment was done to evaluate the glucose utilization capacity of extract. ResultsThe effect of different concentration of ethanolic extract of Acacia Seyal bark on the blood glucose level of diabetic induced rats is significantly different (0.05 p 0.01 <p). The phytochemical screening of extract indicated the presence of Saponin, Tannins Steroids, Triterpens, and Anthraquinone, While the absence of flavonoids. In vitro glucose consumption by diaphragm study the level of utilized glucose from the media is 69.4% in the presence of extract compared to control group. ConclusionStudy concluded that the ethanolic bark extract of A. seyal showed significant anti-diabetic activity. This results might have a great potential for translation to humans and the obtained data might set the stage for clinical trials investigating the effects of anti-diabetic effect in patients with diabetes mellitus type2. In the best of our knowledge, this is the first study that has conducted to study the anti-diabetics effect of ethanolic extract of Acacia Seyal bark in diabetic rats.

pharmacology and toxicology↗

bioRxiv2021

Epitope-Based Peptide prediction of vaccine against European bat lyssaviruses type 2 Glycoprotein G with Immunoinformatic Approach

ObjectiveEuropean bat lyssaviruses (EBLV) type 2 is present in many European countries. Infection is usually seen in bats, the primary reservoirs of the viruses. Human deaths have been documented within few days following bat exposures. So, it is very useful to design an insilco peptide vaccine for European bat lyssaviruses type 2 virus using glycoprotein G as an immunogen to stimulate protective immune response ResultsB cell tests were conducted for Bepipred with 15 conserved epitopes, Emini surface accessibility prediction with 7 conserved epitopes in the surface and Kolaskar and Tongaonkar antigenicity tested with three conserved epitopes being antigenic. 357 conserved epitopes were predicted to interact with different MHC-1 alleles with (IC50) [≤]500 while 282 conserved epitopes found to interact with MHC-II alleles with IC50[≤] 1000. Among all tested epitopes for world population coverage the epitope VFSYMELKV binding to MHC11 alleles was 97.94% and it found to bind 10 different alleles that indicate strong potential to formulate peptide vaccine for lyssaviruses type 2 virus. To the best of our knowledge this is the first study to propose peptide vaccine for European bat lyssavirus type 2.

bioinformatics↗

bioRxiv2020

Epitope-Based Peptide Vaccine Design Against Fructose Bisphosphate Aldolase of Candida Glabrata: An Immunomics Approach

Background Candida glabrata is a human opportunistic pathogen that can cause life-threatening systemic infections. Although, there are multiple effective vaccines against fungal infections, and some of these vaccines were engaged in different stages of clinical trials, none of them yet approved by (FDA).Aim To predict the most conserved and immunogenic B- and T-cell epitopes from the Fructose Bisphosphate aldolase (Fba1) protein of C. glabrata.Materials and Methods 13 C. glabrata Fructose bisphosphate aldolase protein sequences (361amino acid) were retrieved from NCBI and several in silico tools presented in the IEDB server for predicting peptides were used and homology modeling and molecular docking were performed.Result The promising B-cell Epitopes were AYFKPH, VDKESLYTK, and HVDKESLYTK. While, promising peptides which have the high affinity to MHC I binding were: AVHEALAPI, KYFKRMAAM, QTSNGGAAY, RMAAMNQWL and YFKEHGEPL. Two peptides (LFSSHMLDL and YIRSIAPAY) were noted to have the highest affinity to MHC class II that interact with 9 MHC class II alleles. The molecular Docking revealed the epitopes QTSNGGAAY and LFSSHMLDL have the high binding energy to MHC moleculesConclusion The epitope-based vaccines predicted by using immunoinformatics tools have remarkable advantages over the conventional vaccines that they are more specific, less time consuming, safe, less allergic and more antigenic. Further in vivo and in vitro experiments are needed to prove the effectiveness of the best candidates epitopes (QTSNGGAAY and LFSSHMLDL). To the best of our knowledge, this is the first study that has predicted B- and T-cells epitopes from Fba1 protein by using in silico tools in order to design an effective epitope-based vaccine against C. galabrata.Competing Interest StatementThe authors have declared no competing interest.View Full Text

bioinformatics↗