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Biology subjects

Early, A.

Publications and source records attributed to Early, A..

2 recordsLinked to original sources

Classification of Communication and Head Movement Behaviors during Multi-Person Conversations using Deep Learning

Head movements play a pivotal role while engaged in multi-talker conversation by providing non-verbal feedback to partners and enhancing a listeners ability to separate sound sources. Commercial hearing-aids with an on-board IMU (Inertial Measurement Unit, i.e., accelerometers) typically use that information for step-counting and activity levels. At least one device uses it as an input to their environment classifier and integrated directional microphone. None, however, use the IMU to detect specific patterns of head movements to predict behaviors such as nodding, head shaking, listening to a person versus a video, or talking. Training an automatic classifier to accurately detect these behaviors first requires collecting head movement data during multi-person conversation and laboriously annotating each behavior-type for each participant with high temporal precision. From that point, with the goal of training the most accurate model and integrating with the hardware in the hearing aid to improve device performance, the question is how best to model the data. To address this gap, we collected accelerometer data during natural multi-person conversations and paired it with detailed human annotations of communication and head-movement behaviors. Head-movement data was collected from three cohorts of young, normal-hearing individuals (three per cohort) in a controlled, conference-room setting during 50-minute multi-talker conversations. Participants wore hearing aids with on-board accelerometers, and audio-video was recorded for each talker. Videos were manually annotated for communication and head-movement behaviors, including conversational turns and nonverbal cues such as tilts and nods. Temporal and spectral features were extracted from the accelerometer data (windowed into 1-second segments) corresponding to roll and pitch movements. These features, combined with the annotated behaviors, were used to train and test machine learning models. Models were trained on data from all but one participant and then tested on the held-out participant, repeating this procedure across all individuals. Several deep learning and classical machine learning models were compared for classifying communication behaviors (e.g., talking, listening, watching video) and head orientations (e.g., turning left or right, facing down, facing forward). More specifically, various sequence-to-sequence models, a state-of-the-art deep learning technique, were utilized. These models incorporated modern architectural components such as transformer networks. Multiple performance metrics were used to evaluate models, and results suggest that modern deep learning models outperform classical machine learning methods by significant margins. Classification performance improved further when temporal sequence information was incorporated. These results indicate that during multi-talker conversation, hearing-aid accelerometers can automatically classify stereotypical behaviors with high temporal resolution (1-second). Even when tested on unseen subjects, the models remained reliable. This establishes a foundation for more advanced approaches that combine behavioral and movement patterns, further integrate temporal dynamics, and incorporate additional inputs to improve accuracy and ecological validity.

biophysics↗

Cell Cycle Regulation has Shaped Budding Yeast Replication Origin Structure and Function

Eukaryotic DNA replication initiates from multiple genomic loci known as origins. At budding yeast origins like ARS1, a double hexamer (DH) of the MCM replicative helicase is assembled by Origin Recognition Complex (ORC), Cdc6 and Cdt1 via sequential hexamer loading from two opposed ORC binding sites. Cyclin Dependent Kinase (CDK) inhibits DH assembly, which prevents re-replication by restricting helicase loading to G1 phase. Here we show that an intrinsically disordered region (IDR) in the Orc2 subunit promotes interaction between ORC and the first loaded, closed-ring MCM hexamer (the MO intermediate); CDK phosphorylation of this IDR blocks MO formation and DH assembly. We show that MO functions by stabilising ORC at the lower affinity binding sites required for second hexamer loading. Origins comprising two high affinity ORC sites can assemble DH efficiently without MO by independently loading single hexamers; these origins escape CDK inhibition in vitro and in vivo. Our work reveals mechanistic plasticity in MCM loading with implications for understanding how CDK regulation has shaped yeast origin evolution and how natural origins might escape cell cycle regulation. We also identify key steps common to loading pathways, with implications for understanding how MCM is loaded in other eukaryotes.

biochemistry↗