Biology subjects

Dzieciol, M.

Publications and source records attributed to Dzieciol, M..

3 recordsLinked to original sources

bioRxiv2024

The Incisive Duct as a Pathway for Early Vomeronasal Communication in Neonatal Dogs

The detection of chemical signals by the vomeronasal organ (VNO) is critical for communication among mammals from an early age, influencing behaviors such as suckling and recognition of the mother and conspecifics. Located in a concealed position at the base of the nasal cavity, the VNO features a duct covered with a sensory epithelium rich in neuroreceptors. A critical aspect of VNO functionality is the efficient access of stimuli from the nasal and oral cavities to the receptors. In adult dogs, it has been demonstrated through in vivo magnetic resonance imaging and anatomically postmortem how the VNO duct (VD) communicates to the environment through the incisive duct (ID). However, in newborn puppies, the existence of functional communication between the ID and the VD has not been confirmed to date, raising doubts about the potential physiological obliteration of the ID due to its small size and the degree of immaturity of the puppies at birth. Determining this aspect is necessary to evaluate the role played by chemical communication in this critical phase for the survival and socialization of puppies. This study employs serial histological staining techniques to examine the presence and functionality of the incisive duct in neonatal dogs. The serial histological sections have confirmed both the existence of functional communication between both the vomeronasal and incisive ducts in perinatal puppies, and the dual functional communication of the incisive duct with the oral and nasal cavities. The ID shows an uninterrupted lumen along its path and is associated with a sophisticated cartilaginous complex that prevents its collapse, as well as erectile tissue rich in blood vessels and connective tissue that acts as a cushion, facilitating its action under pressure induced by sampling behaviors such as tonguing. This investigation demonstrates the communicative capabilities of the VNO during the perinatal stage in dogs.

neuroscience↗

bioRxiv2019

The sources and transmission routes of microbial populations throughout a meat processing facility

Microbial food spoilage is responsible for a considerable amount of waste and can cause food-borne diseases in humans, particularly in immunocompromised individuals and children. Therefore, preventing microbial food spoilage is a major concern for health authorities, regulators, consumers, and the food industry. However, the contamination of food products is difficult to control because there are several potential sources during production, processing, storage, distribution, and consumption, where microorganisms come in contact with the product. Here, we conduct the first study that uses high-throughput full-length 16S rRNA gene sequencing to provide novel insights into bacterial community structure throughout a pork processing plant. Specifically, we investigated what proportion of bacteria on meat are not animal-associated and are therefore transferred during cutting via personnel, equipment, machines, or the slaughter environment. We then created a facility-specific transmission map of bacterial flow which revealed previously unknown sources of bacterial contamination. This allowed us to pinpoint specific taxa to particular environmental sources and provide the facility with essential information for targeted disinfection. For example, Moraxella spp., a prominent meat spoilage organism which was one of the most abundant amplicon sequence variants (ASVs) detected on the meat, was most likely transferred from the gloves of employees, a railing at the classification step, and the polishing tunnel whips. Finally, we provide evidence that 1000 sequences per sample provides a reasonable sequencing depth for microbial source tracking in food processing, suggesting that this approach could be implemented in regular monitoring systems.

microbiology↗

bioRxiv2019

A prospective role for the rumen in generating antibiotic resistance

Antibiotics were a revolutionary discovery of the 20th century, but the ability of bacteria to spread the genetic determinants of resistance via horizontal gene transfer (HGT) has quickly endangered their use1. Indeed, there is a global network of microbial gene exchange, the analysis of which has revealed particularly frequent transfer of resistance determinants between farm animals and human-associated bacteria2. Here, we leverage the recent release of a rumen microbial genome reference set and show that the wide-spread resistance gene cluster aadE-sat4-aphA-3 is harboured in ruminal Bacteroidetes. While this cluster appears to have been recently transferred between commensal bacteria in the rumen and many diverse animal and human pathogens, comparative analysis suggests that the cluster stabilized in the pathogens. Then, focusing on streptomycin resistance, it was found that homologues from the rumen span much of the known diversity of aminoglycoside O-nucleotidyltransferases (AadEs) and that distinct variants of the enzyme are present in a single rumen bacterial genome. Notably, a second variant of AadE has also been recently transferred, albeit more often as a single gene, throughout a different set of animal and human associated bacteria. By examining the synteny of AadE orthologues in various bacterial genomes and analyzing corresponding gene trees in an environmental context, we speculate that the ruminant associated microbiome has a salient role in the emergence of specific resistance variants and clusters. In light of the recent literature on the evolutionary origin of antibiotic resistance, we further suggest that the rumen provides a possible route of dissemination of resistance genes from soil resistomes, throughout the farm, and to human pathogens3.

microbiology↗