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Biology subjects

Dye, B.

Publications and source records attributed to Dye, B..

2 recordsLinked to original sources

Projected ecosystem responses to environmental changes associated with offshore wind farms and ocean warming

Offshore wind farms (OWFs) are rapidly expanding to meet growing demands for renewable energy, with development expected to extend further offshore into deeper waters. Given this expanded human footprint, it is important to better understand the long-term ecological consequences of OWFs and how these may interact with ongoing climate change. We used the coupled hydrodynamic-ecosystem-biogeochemical water-column model (GOTM-ERSEM-BFM) to investigate ecosystem-wide responses to environmental changes associated with OWFs and climate warming. Specifically, we examined OWF-related scenarios of reduced benthic suspension-feeding activity, representing potential effects of contaminant emissions from OWFs, and reduced wind forcing, together with increased sea surface temperature. The scenarios were simulated individually and in combination to explore potential interactive effects. These scenarios were simulated at two contrasting locations in the North Sea, representing a well-mixed coastal site and a seasonally stratified offshore site. The coastal site exhibited comparatively modest ecosystem responses across the scenarios, whereas responses were generally stronger at the deeper offshore site. At the offshore site, changes in stratification altered vertical nutrient dynamics and contributed to pronounced differences in ecosystem responses between the surface and bottom layers. Our results demonstrate that ecosystem responses to OWF-related and climate-driven environmental changes are strongly dependent on local environmental conditions, suggesting that ecological consequences may differ substantially as wind farm development expands from shallower to deeper environments.

ecology↗

Integrated growth factor signaling promotes lung epithelial progenitor cell expansion and maintenance in mice and humans

The bud tip epithelium of the branching mouse and human lung contains multipotent progenitors that are able to self-renew and give rise to all mature lung epithelial cell types. The current study aimed to understand the developmental signaling cues that regulate bud tip progenitor cells in the human fetal lung, which are present during branching morphogenesis, and to use this information to induce a bud tip progenitor-like population from human pluripotent stem cells (hPSCs) in vitro. We identified that FGF7, CHIR-99021 and RA maintained isolated human fetal lung epithelial bud tip progenitor cells in an undifferentiated state in vitro, and led to the induction of a 3-dimensional lung-like epithelium from hPSCs. 3-dimensional hPSC-derived lung tissue was initially patterned, with airway-like interior domains and bud tip-like progenitor domains at the periphery. Epithelial bud tip-like domains could be isolated, expanded and maintained as a nearly homogeneous population by serial passaging. Comparisons between human fetal lung epithelial bud tip cells and hPSC-derived bud tip-like cells were carried out using immunostaining, in situ hybridization and transcriptome-wide analysis, and revealed that in vitro derived tissue was highly similar to native lung. hPSC-derived epithelial bud tip-like structures survived in vitro for over 16 weeks, could be easily frozen and thawed and maintained multi-lineage potential. Furthermore, hPSC-derived epithelial bud tip progenitors successfully engrafted in the proximal airways of injured immunocompromised NSG mouse lungs, where they persisted for up to 6 weeks and gave rise to several lung epithelial lineages.

developmental biology↗