Dlalah, N. | Search bioRxiv

bioRxiv2024

Identification and Expression of the peach TNL RMia genes for the Resistance to the Root-knot Nematode Meloidogyne incognita

Root-Knot Nematodes (RKN) are major pests for world agriculture. As RKN are polyphagous endoparasites, present in various climates and able to multiply rapidly, the quest to discover new natural resistances in plants is essential. In Prunus species, several major resistance genes to RKN that belong to the NLR family, have been detected in recent years. In Peach, the RMia gene, which confers resistance to the RKN Meloidogyne incognita, M. arenaria, and M. ethiopica, was localized on the chromosome 2. In this study, we used short-and long-read sequencing technologies to identify the RMia gene in two independent RKN-resistant peach varieties. We characterized three candidate genes and studied their expression profile upon RKN infection. Our study identified and characterized these candidates that are most likely involved in the RKN resistance. Ultimately, we developed new molecular markers associated to this resistance that can be used in breeding programs.

plant biology↗

bioRxiv2024

Investigating the genetic architecture of biotic stress response in stone fruit tree orchards under natural infections with a multi-environment GWAS approach

The mapping and introduction of sustainable plant immunity to pests and diseases in fruit tree is still a major challenge in modern breeding. This study aims at deciphering the genetic architecture underlying resistance or tolerance across environments for major pests and diseases in peach (P. persica) and apricot (P. armeniaca). We set up a multi-environment trial (MET) approach by studying two core collections of 206 peach and 150 apricot accessions deployed under low phytosanitary conditions in respectively three and two environmentally contrasted locations in South-East of France. To capture the complex dynamics of pest and disease spread in naturally infected orchards, visual scoring of symptoms was repeated within and between 3 years, for five and two pests and diseases respectively for peach and apricot, resulting in the maximum of damage score and the AUDPC. These traits were used as phenotypic inputs in our genome-wide association studies (GWAS) strategy, and leading to the identification of: i) non-additive genotype-phenotype associations, ii) environment-shared QTLs iii) environment-specific QTLs, and iv) interactive QTLs which changes in direction ( antagonist) or intensity ( differential) according to the environment. By conducting GWAS with multiple methods, we successfully identified a total of 60 high confidence QTLs, leading to the identification of 87 candidate genes, the majority belonging to the Leucine-rich repeat containing receptors (LRR-CRs) family gene. Finally, we provided a comparative analysis of our results on peach and apricot, two closely related species. The present results contribute to the development of genomics-assisted breeding to improve biotic resilience in Prunus varieties.

plant biology↗