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Biology subjects

Dizani, M.

Publications and source records attributed to Dizani, M..

2 recordsLinked to original sources

Programming the Internal Architecture of Synthetic Compartments by Coassembling Filamentous and Liquid DNA Phases

Living cells rely on filaments and condensates as key organizers of their interior. Developing these structural primitives together inside synthetic compartments using programmable components is a step toward building functional synthetic cells, composite biomaterials, and synthetic tissues. Here, we demonstrate that DNA nanotubes and DNA condensates can be co-assembled within cell-sized compartments, including water-in-oil droplets and giant unilamellar vesicles (GUVs). The two nanostructures form as expected, producing a single condensate surrounded by nanotubes in diverse morphologies that depend on DNA and salt concentration, as well as compartment size. By incorporating photoactivatable DNA linkers, we can control the order of assembly and trigger reconfiguration of nanotube networks into ring-shaped bundles enclosing a condensate, architectures reminiscent of a cellular nucleus within a cytoskeletal ring. An isothermal assembly protocol based on monovalent salts further extends this approach to GUVs. Together, these results establish DNA filaments and condensates as programmable, composable organizers of synthetic cell interiors.

synthetic biology↗

Protein recruitment to dynamic DNA-RNA host condensates

We describe the design and characterization of artificial nucleic acid condensates that are engineered to recruit and locally concentrate proteins of interest in vitro. These condensates emerge from the programmed interactions of nanostructured motifs assembling from three DNA strands and one RNA strand that can include an aptamer domain for the recruitment of a target protein. Because condensates are designed to form regardless of the presence of target protein, they function as "host" compartments. As a model protein we consider streptavidin (SA) due to its widespread use in binding assays, thus the host condensates presented here could find immediate use for the physical separation of a variety of biotin-tagged components. In addition to demonstrating protein recruitment, we describe two approaches to control the onset of condensation and protein recruitment. The first approach uses UV irradiation, a physical stimulus that bypasses the need for exchanging molecular inputs and is particularly convenient to control condensation in emulsion droplets. The second approach uses RNA transcription, a ubiquitous biochemical reaction that is central to the development of the next generation of living materials. We finally show that the combination of RNA transcription and degradation leads to an autonomous dissipative system in which host condensates and protein recruitment occur transiently, and that the host condensate size as well as the timescale of the transient can be controlled by the level of RNA degrading enzyme. For Table of Contents Only O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=109 SRC="FIGDIR/small/597281v1_ufig1.gif" ALT="Figure 1"> View larger version (22K): org.highwire.dtl.DTLVardef@1f6dc00org.highwire.dtl.DTLVardef@1e5a9eforg.highwire.dtl.DTLVardef@f7c247org.highwire.dtl.DTLVardef@1f3c3d0_HPS_FORMAT_FIGEXP M_FIG C_FIG

synthetic biology↗