Dierksheide, K. J. | Search bioRxiv

bioRxiv2025

Canonical transcription termination mechanisms explain a minority of operons in cyanobacteria

Cyanobacteria are the most abundant phototrophs and hold potential as a carbon-negative platform for bioengineering applications. However, these efforts have been hampered by limited mechanistic understanding of their gene expression, including transcription termination. Unlike most bacteria, cyanobacteria lack the transcription termination factor Rho, raising the speculation that all transcription ends with intrinsic terminators. Here we show that most transcription units (TUs) in Synechococcus elongatus PCC 7942 are not terminated by known termination pathways. Although many TUs (52%) have unique, well-defined 3' ends, only a small fraction have features that resemble canonical intrinsic terminators (22%). The noncanonical 3' ends broadly lacked strong secondary structure, making it unclear how these ends are protected against 3'-5' exonucleolytic decay. Furthermore, many TUs (46%) have diverse positions of mRNA 3' ends, suggesting a potentially diffuse termination signal. Finally, we observed a moderate increase in RNA levels downstream of most defined 3' ends in the absence of the transcription-repair coupling factor Mfd. This finding indicates that Mfd plays a limited, but widespread, role in RNA end formation, potentially through termination of stalled RNAPs. Together, our work reveals unique end architectures of the cyanobacterial transcriptome and suggests that undescribed transcription termination mechanisms are active in the phylum. ImportanceOur understanding of bacterial transcription regulation is largely based on model organisms like Escherichia coli and Bacillus subtilis, yet many of these mechanisms appear absent or divergent in cyanobacteria. These differences limit our fundamental understanding of gene regulation and the applied potential of cyanobacteria in sustainable biomanufacturing. To address this gap, we characterized transcription termination in the model cyanobacterium Synechococcus elongatus PCC 7942. We resolve a longstanding question by showing that intrinsic termination alone cannot account for most termination events in this organism. Pervasive transcript ends lacking intrinsic terminator features and the absence of Rho suggest the existence of novel termination mechanism(s) and highlight a largely unexplored regulatory landscape. Simultaneously, our work expands the repertoire of functionally characterized cyanobacterial intrinsic terminators, offering a new toolkit to fine-tune gene expression using terminators of defined strengths. These findings pave the way for more predictable and powerful applications of cyanobacteria in green biotechnology.

microbiology↗

bioRxiv2025

Widespread purine bias in bacterial genes driven by runaway transcription

Genes in many bacteria are rich in purine nucleotides and poor in pyrimidines. We show that this purine preference is critical for gene expression because it prevents premature transcription termination in species that exhibit runaway transcription. In contrast to coupled transcription-translation1-5, runaway RNA polymerases that outpace trailing ribosomes have exposed nascent RNA and are vulnerable to the termination factor Rho6,7. Using a massively parallel reporter assay in Bacillus subtilis, we found that Rho-dependent termination requires a high C-to-G skew and high T content. Consequently, purine-rich coding (sense) sequences escape premature termination, whereas the correspondingly pyrimidine-rich antisense sequences are targeted by Rho and transcriptionally silenced. This purine requirement drives biased codon usage in most bacterial species with runaway transcription, except in lineages that have lost Rho. Our results suggest that the avoidance of premature transcription termination imposes major constraints on nucleotide content during genome evolution and adaptation of foreign genes.

microbiology↗

bioRxiv2024

A historical sequence deletion in a commonly used Bacillus subtilis chromosome integration vector generates undetected loss-of-function mutations

Since the 1980s, chromosome-integration vectors have been used as a core method of engineering Bacillus subtilis. One of the most frequently used vector backbones contains chromosomally derived regions that direct homologous recombination into the amyE locus. Here, we report a gap in the homology region inherited from the original amyE integration vector, leading to erroneous recombination in a subset of transformants and a loss-of-function mutation in the downstream gene. Internal to the homology arm that spans the 3' portion of amyE and the downstream gene ldh, an unintentional 227-bp deletion generates two crossover events. The major event yields the intended genotype, but the minor event, occurring in [~]10% of colonies, results in a truncation of ldh, which encodes lactate dehydrogenase. Although both types of colonies test positive for amyE disruption by starch plating, the potential defect in fermentative metabolism may be left undetected and confound the results of subsequent experiments.

microbiology↗