Search bioRxiv⌕ Search

Biology subjects

Diaz, S. M.

Publications and source records attributed to Diaz, S. M..

2 recordsLinked to original sources

Functional diversity of a temperate continental flora: Wide but skewed coverage of the global spectrum of plant form and function

O_LIHabitat filtering is assumed to promote plant functional convergence, but local functional ranges are often wide. Using the Global Spectrum of Plant Form and Function (GSPFF) as global reference, we tested whether a regional flora shared the same trait trade-offs as the global flora, and whether the regional phenospace supports the habitat filtering hypothesis. C_LIO_LIWe measured six key functional traits in 268 central Argentina species. We compared this flora with the GSPFF using univariate and multivariate methods. We tested whether the regional phenospace was smaller or more skewed than expected by chance, and analysed its topography. C_LIO_LIRegional and global phenospaces shared major axes, syndromes, and trait correlations. Despite having fewer species, the regional flora broadly covered the GSPFF, but without tall, large-leaved, large-seeded trees. The most frequent species were conservative herbs and shrubs. Distinct species also showed highly conservative attributes. C_LIO_LIDespite being moderate to harsh, central Argentina environmental conditions do not appear to preclude the regional presence of most functional syndromes previously identified at the global scale. However, the regional and global floras differ in the most frequent syndromes. At the regional scale, habitat filtering shapes functional topographies more than it constrains the extent of phenospaces. C_LI

ecology↗

Antigenic mapping and functional characterization of human New World hantavirus neutralizing antibodies

Hantaviruses are high-priority emerging pathogens carried by rodents and transmitted to humans by aerosolized excreta or, in rare cases, person-to-person contact. While sporadic in North and South America, many infections occur in Europe and Asia, with mortality ranging from 1 to 40% depending on the hantavirus species. There are currently no FDA-approved vaccines or therapeutics for hantaviruses, and the only treatment for infection is supportive care for respiratory or kidney failure. Additionally, the humoral immune response to hantavirus infection is incompletely understood, especially the location of major antigenic sites on the viral glycoproteins and conserved neutralizing epitopes. Here, we report antigenic mapping and functional characterization for four neutralizing hantavirus antibodies. The broadly neutralizing antibody SNV-53 targets an interface between Gn/Gc, neutralizes through fusion inhibition and cross-protects against the Old World hantavirus species Hantaan virus when administered pre- or post-exposure. Another broad antibody, SNV-24, also neutralizes through fusion inhibition but targets domain I of Gc and demonstrates weak neutralizing activity across hantavirus species. ANDV-specific, neutralizing antibodies (ANDV-5 and ANDV-34) neutralize through attachment blocking and protect against hantavirus cardiopulmonary syndrome (HCPS) in animals but target two different antigenic faces on the head domain of Gn. Determining the antigenic sites for neutralizing antibodies will contribute to further therapeutic development for hantavirus-related diseases and inform the design of new broadly protective hantavirus vaccines.

immunology↗