Biology subjects

Di, T.

Publications and source records attributed to Di, T..

3 recordsLinked to original sources

bioRxiv2026

Sleep reactivation shapes memories of event and its temporal structure

Episodic memory provides a temporal scaffold that organizes past experiences according to their sequential occurrence. While memories are reactivated and consolidated during sleep, it remains unknown how exogenous reactivation reshapes memories of complex events, particularly the temporal structure that binds individual elements across time. In this preregistered study, 35 participants encoded neutral and aversive narratives composed of image sequences, with auditory context shifts defining event boundaries. During post-encoding slow-wave sleep, closed-loop targeted memory reactivation (TMR) delivered boundary-associated auditory cues time-locked to the up-state of slow oscillations. Reactivation led to faster, more confident, and more liberal recognition responses for cued than uncued memories. However, reactivation exerted a time-dependent effect on temporal-order memory, impairing it immediately after sleep but attenuating forgetting one week later. This behavioral dissociation was paralleled at the neural level: stronger cue-elicited theta activity predicted superior recognition of neutral, but not aversive event content, whereas greater theta responses were associated with poorer temporal-order memory for the same events. Together, these findings suggest that sleep reactivation does not uniformly strengthen episodic memories. Rather, it selectively prioritizes access to event content while reorganizing temporal relations among event elements, revealing distinct, time-dependent consequences of sleep reactivation for different components of complex experiences.

neuroscience↗

bioRxiv2025

Abnormally increased intrinsic neural timescales in sensory and default mode networks in cocaine use disorder

Cocaine use disorder (CUD) is associated with abnormal structural and functional brain changes. However, the neurodynamics and molecular underpinnings remain unclear. In this study, we mapped whole-brain intrinsic neural timescales (INTs), reflecting temporal neural processing, using resting-state functional magnetic resonance imaging data from 44 CUD patients and 44 healthy controls (HC). CUD showed increased INTs in visual, somatomotor, and default mode networks compared with HC. Mediation analysis linked local INTs abnormalities to altered dorsal attention network neurodynamics, associated with inhibitory control deficits. Notably, these changes were primarily correlated with alterations in gamma-aminobutyric acid type A receptors and the noradrenaline transporter. Machine learning classifiers based on INTs achieved a maximum accuracy of 75.5% in distinguishing CUD from HC, with a generalization accuracy of 65.0% on an independent dataset. This study elucidates aberrant neural mechanisms underlying CUD and highlights INTs as promising diagnostic biomarkers for clinical detection and intervention. TeaserSpatiotemporal neuroscience reveals intrinsic neural timescale disruptions underlying cocaine use disorder, offering novel diagnostic biomarkers.

neuroscience↗

bioRxiv2022

The tea plant (Camellia sinensis) FLOWERING LOCUS C-like gene CsFLC1 is correlated with bud dormancy and triggers early flowering in Arabidopsis

Flowering and bud dormancy are crucial stages in the life cycle of perennial angiosperms in temperate climates. MADS-box family genes are involved in many plant growth and development processes. Here, we identified 3 MADS-box genes in tea plant belonging to the FLOWERING LOCUS C (CsFLC) family. We monitored CsFLC1 transcription throughout the year and found that CsFLC1 was expressed at a higher level during the winter bud dormancy and flowering phases. To clarify the function of CsFLC1, we developed transgenic Arabidopsis thaliana plants heterologously expressing 35S::CsFLC1. These lines bolted and bloomed earlier than the WT (Col-0), and the seed germination rate was inversely proportional to the increased CsFLC1 expression level. RNA-seq of 35S::CsFLC1 transgenic Arabidopsis showed that many genes responding to ageing, flower development and leaf senescence were affected, and phytohormone-related pathways were especially enriched. According to the results of hormone content detection and RNA transcript level analysis, CsFLC1 controls flowering time possibly by regulating SOC1, AGL42, SEP3 and AP3 and hormone signalling, accumulation and metabolism. Our results suggest that CsFLC1 might play dual roles in flowering and winter bud dormancy and provide new insight into the molecular mechanisms of FLC in tea plants as well as other plant species. HighlightThree FLOWERING LOCUS C-like genes were identified in tea plants, among them CsFLC1 played dual roles in flowering and winter bud dormancy.

plant biology↗