Search bioRxiv⌕ Search

Biology subjects

Dehkordi, M.

Publications and source records attributed to Dehkordi, M..

2 recordsLinked to original sources

ArchMap: A web-based platform for reference-based analysis of single-cell datasets

Leveraging single cell reference atlases to analyse new data has brought about a paradigm shift in single cell data science akin to the first reference genome in genomics. However methods to perform this mapping require computational expertise as well as sometimes considerable compute power, and thus may exclude the researchers from this innovation who may benefit the most from it. ArchMap, a no-code query-to-reference mapping tool, removes this barrier by providing all-in-one automated mapping, cell type annotation, and collaborative features to analyse single-cell datasets from a wide range of integrated, often published, reference atlases and allows extension of atlases with the growing Human Cell Atlas and related efforts. This paves the way for a democratisation of reference mapping capabilities.

bioinformatics↗

Mera: A scalable high throughput automated micro-physiological system

There is an urgent need for scalable Microphysiological Systems (MPSs)1 that can better predict drug efficacy and toxicity at the preclinical screening stage. Here we present Mera, an automated, modular and scalable system for culturing and assaying microtissues with interconnected fluidics, inbuilt environmental control and automated image capture. The system presented has multiple possible fluidics modes. Of these the primary mode is designed so that cells may be matured into a desired microtissue type and in the secondary mode the fluid flow can be re-orientated to create a recirculating circuit composed of inter-connected channels to allow drugging or staining. We present data demonstrating the prototype system Mera using an Acetaminophen/HepG2 liver microtissue toxicity assay with Calcein AM and Ethidium Homodimer (EtHD1) viability assays. We demonstrate the functionality of the automated image capture system. The prototype microtissue culture plate wells are laid out in a 3 x 3 or 4 x 10 grid format with viability and toxicity assays demonstrated in both formats. In this paper we set the groundwork for the Mera system as a viable option for scalable microtissue culture and assay development.

bioengineering↗