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DeCurtis, E.

Publications and source records attributed to DeCurtis, E..

2 recordsLinked to original sources

Characterization of an immunodeficiency-associated EZH2 variant

Regulation of gene expression is central to the development of immune cells and their ability to respond to infection. As part of a clinical evaluation, we identified two sisters with recurrent infections, hypogammaglobulinemia, and memory B cell deficiency, diagnosed as common variable immunodeficiency. Whole exome sequencing identified a heterozygous variant (Leu50Ser, L50S) in a conserved region of EZH2, the catalytic subunit of the epigenetic gene repressor Polycomb Repressive Complex 2 (PRC2). EZH2-catalyzed histone H3 lysine 27 methylation (H3K27me) in bulk was not overall significantly disrupted by this variant, in patient samples or cell lines expressing EZH2-L50S. EZH2-L50S protein is expressed similar to wild-type and can form PRC2. However, we find that specific genomic regions that normally have high wild-type levels of H3K27me3 are deficient in the L50S context, particularly around gene promoters. EZH2-L50S is still recruited to these sites, but is not as active. Using recombinant purified PRC2, we determine that L50S affects methylation of nucleosomes and disrupts allosteric stimulation that normally amplifies H3K27me3, consistent with the location of L50 in the allosteric regulatory region of PRC2. Thus, variation of EZH2 L50, occurring at low frequency in the population may interfere with normal B cell gene expression patterns, contributing to immunodeficiency. This study has implications for genetic variation in PRC2 in the general population.

molecular biology↗

Characterization of the Oral Bacteriome of the Healthy Lewis Rat

Rodents are the most frequently utilized animals for in vivo research, and rats are the most abundant rodent used to study peri-implantitis disease and progression. However, there are very few studies available that elucidate the healthy oral microbiome of rats. The aim of this study was to characterize the healthy oral bacteriome of the male Lewis rat at two unique niches, the gums and first molar, by sequencing the 16S rDNA v1-v3 region. We collected the microbiota of 12 male Lewis rats at the toothless alveolar crest and the first molar, on both sides of the mouth. We identified an average of 20,820 sequences per sample. There were no significant differences between the rat groups or sample sites in the diversity ( or {beta}) of the bacteriome. A total of 359 amplicon sequence variants (ASVs) were identified in the bacteriome, correlating to the phyla Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. Parallels exist at the genus level between human and rat oral bacteriomes, however, the overall diversity between comparable sites is lower in rats than humans. Similar findings were reached when using pooled or individual swab samples of rat mouths. This study characterizes two unique ecological niches within the healthy rat oral bacteriome and provides a baseline for comparison with future studies. IMPORTANCERats are frequently used for dental research, but little is known about their normal oral microbiota. Here, we use DNA sequence analysis to identify bacterial groups colonizing the rat mouth at the tooth surface and at the gums. This work contributes to our knowledge of bacterial diversity in the rat mouth.

microbiology↗