Biology subjects

Dal Toe, L.

Publications and source records attributed to Dal Toe, L..

3 recordsLinked to original sources

bioRxiv2026

Mammalian condensin I controls higher-order chromosome organization and homologous recombination in meiotic prophase I

The meiotic chromosome structure, defined by the axis-chromatin loop organization, is essential for proper homologue synapsis through the formation of the synaptonemal complex, and faithful homologous recombination. Here, we show that the conserved SMC-condensin I complex, which shapes the genome through chromatin loop extrusion, plays a pivotal role in controlling the higher-order prophase I meiotic chromosome structure. By analyzing the NCAPD2 condensin I subunit, we found that this complex colocalizes with prophase I chromatin and is essential for male fertility. NCAPD2 restricts chromatin loop extension and controls the localization of chromosome structural proteins, including the axial component HORMAD1 and central element proteins (SYCP1, TEX12, SIX6OS1), thus regulating the synaptonemal complex width. Moreover, NCAPD2 promotes timely and efficient recombination by controlling {gamma}H2AX, DMC1 and pro-crossover proteins turnover. We propose that condensin I, by organizing chromatin loops in prophase I, controls synaptonemal complex organization and recombination outcome in mammals.

genetics↗

bioRxiv2025

HEIP1 orchestrates pro-crossover protein activity during mammalian meiosis

Meiotic crossovers are needed to produce genetically balanced gametes. In mammals, crossover formation is mediated by a conserved set of pro-crossover proteins via mechanisms that remain unclear. Here, we characterize a mammalian pro-crossover factor HEIP1. In mouse HEIP1 is essential for crossing over and fertility of both sexes. HEIP1 promotes crossing over by orchestrating the recruitment of other pro-crossover proteins, including the MutS{gamma} complex (MSH4- MSH5) and E3 ligases (HEI10, RNF212, and RNF212B), that are required to mature crossover sites and recruit the crossover-specific resolution complex MutL{gamma}. Moreover, HEIP1 directly interacts with HEI10, suggesting a direct role in controlling the recruitment of pro-crossover E3 ligases. During early stages of meiotic prophase I, HEIP1 interacts with the chromosome axes, independently of recombination, before relocalizing to the central region of the synaptonemal complex. We propose that HEIP1 is a new conserved master regulator of crossover proteins that controls different crossover maturation steps. Significance StatementCrossovers are essential to produce gametes by promoting the proper segregation of the homologous chromosomes. But, if misregulated, they can lead to genetic disorders, miscarriage and infertility. Their formation depends on the conserved pro-crossover factors, which repertoire is expanding and mode of action tightly regulated. This study highlights how the HEIP1 protein organizes pro-crossover protein activities in the mouse. Our findings show that HEIP1, by interacting early with chromosomes independently of recombination initiation, and orchestrating the recruitment of pro-crossover factors, including the MutSg complex and the RING proteins HEI10, RNF212 and RNF212B, is pivotal in this regulation. Our work is of significance to unravel crossover control during meiotic recombination, a conserved mechanism essential for gametes formation.

genetics↗

bioRxiv2024

Transcriptomic analysis of meiotic genes during the mitosis-to-meiosis transition in Drosophila females

Germline cells produce gametes, which are specialized cells essential for sexual reproduction. Germline cells first amplify through several rounds of mitosis before switching to the meiotic program, which requires specific sets of proteins for DNA recombination, chromosome pairing and segregation. Surprisingly, we previously found that some proteins of the synaptonemal complex, a prophase I meiotic structure, are already expressed and required in the mitotic region of Drosophila females. Here, to assess if additional meiotic genes were expressed earlier than expected, we isolated mitotic and meiotic cell populations to compare their RNA content. Our transcriptomic analysis reveals that all known meiosis I genes are already expressed in the mitotic region, however, only some of them are translated. As a case study, we focused on mei-W68, the Drosophila homologue of Spo11, to assess its expression at both the mRNA and protein levels, and used different mutant alleles to assay for a pre-meiotic function. We could not detect any functional role for Mei-W68 during homologous chromosome pairing in dividing germ cells. Our study paves the way for further functional analysis of meiotic genes expressed in the mitotic region. Article SummaryGermline cells, crucial for sexual reproduction, were thought to switch to meiosis only after several rounds of mitosis. Surprisingly, a few meiotic proteins were found active in the mitotic phase of female flies. Here, we discovered that all known meiosis genes were expressed during mitosis, but only some produced proteins. This study suggests that genes related to reproduction are active earlier than expected, prompting further exploration into their functions during early cell division.

developmental biology↗