Biology subjects

Coulibaly, S.

Publications and source records attributed to Coulibaly, S..

3 recordsLinked to original sources

bioRxiv2024

Sortilin C-terminal fragment deposition depicts tangle-related nonamyloid neuritic plaque growth in Alzheimers disease

Sortilin C-terminal fragments (sorfra) can co-deposit in {beta}-amyloid (A{beta}) plaques in human brain. However, sorfra plaques develop in the cerebrum with a spatiotemporal trajectory as of tauopathy. Here we examined sorfra pathogenesis relative to neuritic plaque evolution in the human brains with amyloid and tau pathologies converged in the neocortex and hippocampus. Sorfra plaques occurred in correlation with pTau/tangle, but not A{beta}, pathologies across cerebral regions, neighboring cortical/hippocampal areas, and along the sulcal valley to gyral hilltop transition. Sorfra plaques and neuritic plaques were matchable in location, shape and size between consecutive sections, and were colocalized in double-labeling preparations. Microscopical study and tissue clearance three-dimensional imaging revealed sorfra/A{beta} colocalized as well as independent plaques. Among the former, sorfra labeling correlated negatively to A{beta}/amyloid labeling and {beta}-secretase-1 labeling in dystrophic neurites. Sorfra plaques were depleted of microtubule-associated protein 2 (MAP2) labeled neuronal somata and dendrites, whereas normal looking MAP2/sortilin co-labeled profiles occurred nearby. Sorfra deposits were seen in astrocytes but not microglia around the plaques. Taken together, sorfra plaques are anatomically matchable to silver stained neuritic plaques. They develop with tangle-related somatodendritic degeneration, presenting as nonamyloid growth of the A{beta} plaques and formation of A{beta}-independent neuritic plaques during Alzheimers disease pathogenesis.

neuroscience↗

bioRxiv2022

Assessment of Culicidae collection methods for xenomonitoring lymphatic filariasis in malaria co-infection context in Burkina Faso

BackgroundEntomological surveillance of lymphatic filariasis (LF) and malaria infections play an important role in the decision-making of national programs, to control or eliminate these both diseases. In order to corroborate infections in the human population, sampling large numbers of mosquitoes is necessary. To overcome this challenge, this study was design to assess the efficiency of four mosquito collection methods for monitoring LF and malaria infections in mosquito population. Methodology/Principle FindingsMosquito collections were performed between August and September 2018 in four villages (Koulpissy, Seiga, and Peribgan, Saptan), distributed in East and South-West health regions of Burkina Faso. Different collection methods were used: Human Landing Catches (HLC) executed indoor and outdoor, Window Exit-Trap, Double Net Trap (DNT) and Pyrethrum Spray Catches (PSC). Molecular analyses were performed to identify Anopheles gambiae s.l. sibling species and to detect Wuchereria bancrofti and Plasmodium falciparum infection in mosquitoes. A total of 3,322 mosquitoes were collected among this, Anopheles gambiaes.l. was the vector caught in largest proportion (63.82%). An. gambiae s.l. sibling species molecular characterization showed that Anopheles gambiae was the dominant specie in all health regions. The Human Landing Catches (indoor and outdoor) collected the highest proportion of mosquitoes (between 61.5%and 82.79%). For sampling vectors infected to W. bancrofti and P. falciparum, PSC, HLC and Window Exit-Trap were been find as the most effective collection methods. Conclusions/SignificanceThis study revealed that HLC indoor and outdoor remained the most effective collection methods. Likewise, the results showed the probability to use Window Exit-Trap and PSC collection methods to sample Anopheles infected and can be useful for xenomonitoring for both LF and malaria. Author summaryIn Burkina Faso the monitoring and evaluation scheme to assess the impact of LF and malaria interventions is only focusing on parasitological tests. While nowadays, the most simple and direct measure of vector borne diseases is xenomonitoring. Thus, in order to confirm both diseases infection rate in the human population, sampling large numbers of mosquitoes is necessary. This study was undertaken in this context to assess the efficiency of four mosquito collection methods for xenomonitoring LF and malaria. Mosquito collections were performed between August and September 2018 in four villages, distributed in East and South-West health regions of Burkina Faso. Human Landing Catches (HLC), Window Exit-Trap, Double Net Trap (DNT) and Pyrethrum Spray Catches (PSC) were evaluated. The results showed that HLC remained the most effective collection method by collecting the highest number of Anopheles (2,388; 71.88% of total). Across the study, mosquito infection rate for Wuchereria bancrofti and Plasmodium falciparum were 0.004 and 0.13 respectively. To collect vectors infected it was found that Window Exit-Trap and PSC were efficiencies. In conclusion, HLC has shown to be appropriate for collect large number of mosquitoes. Likewise, Window Exit-Trap and PSC can be useful for xenomonitoring for both LF and malaria.

pathology↗

bioRxiv2019

Anopheles bionomic, insecticide resistance and malaria transmission in southwest Burkina Faso: a pre-intervention study

BackgroundThe present study presents results of entomological surveys conducted to address the malaria vectors bionomic, insecticide resistance and transmission prior to the implementation of new strategies complement long-lasting insecticidal nets (LLINs) in the framework of a randomized control trial in southwest Burkina Faso. MethodsWe conducted entomological surveys in 27 villages during the dry cold season (January 2017), dry hot season (March 2017) and rainy season (June 2017). We carried out hourly catches (from 17:00 to 09:00) inside and outside 4 houses in each village using the Human Landing Catch technique. Mosquitoes were identified using morphological taxonomic keys. Specimens belonging to the Anopheles gambiae complex and Funestus Group were identified using molecular techniques as well as detection of Plasmodium falciparum infection and insecticide resistance target-site mutations. ResultsEight Anopheles species were detected in the area. Anopheles funestus s.s was the main vector during the dry cold season. It was replaced by Anopheles coluzzii during the dry hot season whereas An. coluzzii and An. gambiae s.s. were the dominant species during the rainy season. Species composition of the Anopheles population varied significantly among surveys. All researched target site mutation of insecticide resistance (kdr-w, kdr-e and ace-1) were detected in all members of the An. gambiae complex of the area but at different frequencies. We observed early and late biting phenotypes in the main malaria vector species. Entomological inoculation rates were 0.087, 0.089 and 0.375 infected bites per human per night during dry cold season, dry hot season and rainy season, respectively. ConclusionThe intensity of malaria transmission was high despite the universal coverage with LLINs. We detected early and late biting phenotypes in the main malaria vector species as well as physiological insecticide resistance mechanisms. These vectors might mediate residual transmission. These data highlight the need to develop complementary tools in addition to LLINs in order to better control resistant malaria vectors and to monitor insecticide resistance.

epidemiology↗