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Cohn, A.

Publications and source records attributed to Cohn, A..

2 recordsLinked to original sources

Getting stuck in a rut as an emergent feature of a dynamic decision-making system

Human sensorimotor decision-making has a tendency to get stuck in a rut, being biased towards selecting a previously implemented action structure ( hysteresis). Existing explanations cannot provide a principled account of when hysteresis will occur. We propose that hysteresis is an emergent property of a dynamical system learning from the consequences of its actions. To examine this, 152 participants moved a cursor to a target on a tablet device whilst avoiding an obstacle. Hysteresis was observed when the obstacle moved sequentially across the screen between trials, but not with random obstacle placement. Two further experiments (n = 20) showed an attenuation when time and resource constraints were eased. We created a simple computational model capturing dynamic probabilistic estimate updating that showed the same patterns of results. This provides the first computational demonstration of how sensorimotor decision-making can get stuck in a rut through the dynamic updating of its probability estimates. Significance StatementHumans show a bias to select the organisational structure of a recently carried out action, even when an alternative option is available with lower costs. This hysteresis is said to be more efficient than creating a new plan and it has been interpreted as a design feature within decision-making systems. We suggest such teleological arguments are redundant, with hysteresis being a naturally emergent property of a dynamic control system that evolved to operate effectively in an uncertain and partially observable world. Empirical experimentation and simulations from a first principle computational model of decision-making were consistent with our hypothesis. The identification of such a mechanism can inform robotics research, suggesting how robotic agents can show human-like flexibility in complex dynamic environments.

neuroscience↗

The Typhoid Toxin Produced by the Nontyphoidal Salmonella Serovar Javiana Can Utilize Multiple Binding Subunits, which Compete for Inclusion in the Holotoxin

Salmonella enterica encodes a wide array of virulence factors. One novel virulence factor, a DNA-damaging toxin known as the typhoid toxin (TT), was recently characterized in >40 nontyphoidal Salmonella (NTS) serovars. Interestingly, these NTS serovars, including S. enterica subsp. enterica serovar Javiana, also encode artB, a homolog of the binding subunit (PltB) of the TT. Here, we show that ArtB and PltB compete for inclusion in the pentameric binding subunit of the TT. Using a combination of in silico modeling, a bacterial two-hybrid system expressed in S. Javiana, and tandem affinity purification (TAP) of the holotoxin subunits, we show that ArtB and PltB interact in vivo. Furthermore, binding subunits composed of homo- and heteropentamers of ArtB and PltB are able to associate with CdtB and PltA to form biologically active toxins. As artB was, (i) conserved among S. Javiana isolates, and (ii) co-expressed with pltB and cdtB under Mg2+-limiting conditions, we hypothesized that ArtB and PltB compete for inclusion in the binding subunit. Using a novel competition assay, we show that PltB outcompetes ArtB for inclusion in the binding subunit, when cultured at neutral pH. Together, our results suggest that the TT produced by S. Javiana utilizes multiple configurations of the binding subunit, representing a novel toxin form and adaptation mechanism for the AB5 toxin family. Our work suggests that Salmonella serovars, including S. Javiana, evolved to encode and maintain multiple binding subunits that can be used to form an active toxin, which may enhance the variety of cells, tissues, or hosts susceptible to this novel form of the TT.

microbiology↗