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Cid-Samper, F.

Publications and source records attributed to Cid-Samper, F..

2 recordsLinked to original sources
bioRxiv2018

Xist lncRNA forms silencing granules that induce heterochromatin formation and repressive complexes recruitment by phase separation

Main text Main text Microscopy RNA Structure Protein-RNA interactions Granule propensity Material and methods in... References Long non-coding RNAs (lncRNAs) are RNA molecules longer than 200 bases that lack coding potential1,2. They represent a significant portion of the cell transcriptome3 and work as activators or repressors of gene transcription acting on different regulatory mechanisms4-6. Indeed, lncRNAs can act as macro-scaffolds for protein recruitment7-14 and behave as guides and sponges for titrating RNA and proteins, influencing transcription at regulatory regions or triggering transcriptional interfere ...

molecular biology↗
bioRxiv2018

Phase Separations Driven by RNA Scaffolds and Protein Sequestration in FXTAS

Recent evidence indicates that specific RNAs promote formation of ribonucleoprotein condensates by acting as scaffolds for RNA-binding proteins (RBPs).\n\nWe systematically investigated RNA-RBP interaction networks to understand ribonucleoprotein assembly. We found that highly-contacted RNAs are highly structured, have long untranslated regions (UTRs) and contain nucleotide repeat expansions. Among the RNAs with such properties, we identified the FMR1 3 UTR that harbors CGG expansions implicated in Fragile X-associated Tremor/Ataxia Syndrome (FXTAS).\n\nWe studied FMR1 binding partners in silico and in vitro and prioritized the splicing regulator TRA2A for further characterization. In a FXTAS cellular model we validated TRA2A-FRM1 interaction and investigated implications of its sequestration at both transcriptomic and post-transcriptomic levels. We found that TRA2A co-aggregates with FMR1 in a FXTAS mouse model and in post mortem human samples.\n\nOur integrative study identifies key components of ribonucleoprotein aggregates, providing links to neurodegenerative disease and allowing the discovery of new therapeutic targets.

molecular biology↗